Chromosome counts on the fern genus Gymnocarpium (Dryopteridaceae) from Finland
Chromosome counts on the fern genus Gymnocarpium (Dryopteridaceae) from Finland
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来自芬兰的蕨类植物 Gymnocarpium（鳞毛蕨科）的染色体计数
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
P. Uotila
中科院分区：
文献类型：
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作者：
K. Pellinen;J. Sarvela;P. Uotila
Carnoy液（甲醇-氯仿-乙酸，6：3：1）。根据以往的经验，第一次减数分裂发生在这个阶段。将材料储存在-25 ° C的固定剂中。随后，用福尔根法对样品进行染色，并将其压扁。挤压的材料用乙酰苏木精染色。用Wild M20显微镜对中期Ⅰ的孢子母细胞进行染色体计数。
by Carnoy liquid (methanol–chloroform–acetic acid, 6:3:1). According to the previous experience, the first meiotic divisions take place at this phase. The material was stored in the fixative at –25°C. Later the samples were stained by the feulgen method, and squashed. Squashed material was stained by aceto-hematoxylin. The chromosome counts were made from the spore mother cells at metaphase I by using a Wild M20 microscope.