Tissue engineering of articular cartilage using an allograft of cultured chondrocytes in a membrane-sealed atelocollagen honeycomb-shaped scaffold (ACHMS scaffold)
Tissue engineering of articular cartilage using an allograft of cultured chondrocytes in a membrane-sealed atelocollagen honeycomb-shaped scaffold (ACHMS scaffold)
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DOI：
10.1002/jbm.b.30284
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Nemoto, K
中科院分区：
文献类型：
--
作者：
Masuoka, K;Asazuma, T;Nemoto, K
本研究的目的是调查与组织工程程序的可能性，使用去端胶原蜂窝状支架密封与膜（ACHMS支架）的软骨细胞培养，以修复关节软骨缺损。将来自日本白色兔关节软骨的软骨细胞在ACHMS支架中培养，以允许高密度三维培养长达21天。尽管支架中的DNA含量以低于单层培养的速率增加，但扫描电子显微镜数据显示，支架在21天后充满了生长的软骨细胞及其产生的细胞外基质。此外，支架培养物中的糖胺聚糖（GAG）积累水平高于单层培养物。体外培养14天的软骨显示出足够的弹性和刚度，可以在体内处理。在兔股骨的髌骨沟中开始关节软骨缺损，随后用软骨细胞培养的ACHMS支架、单独的ACHMS支架或未填充的（对照）填充。手术后三个月，组织学分析显示，只有插入有在ACHMS支架中培养的软骨细胞的缺损被修复性透明软骨填充，从而高度表达II型胶原。这些结果表明，同种异体软骨细胞在ACHMS支架中培养的植入可以有效地修复关节软骨缺损。(c)2005 Wiley Periodicals，Inc.
The aim of this study was to investigate with tissue engineering procedures the possibility of using atelocollagen honeycomb-shaped scaffolds sealed with a membrane (ACHMS scaffold) for the culturing of chondrocytes to repair articular cartilage defects. Chondrocytes from the articular cartilage of Japanese white rabbits were cultured in ACHMS scaffolds to allow a high-density, three-dimensional culturing for up to 21 days. Although the DNA content in the scaffold increased at a lower rate than monolayer culturing, scanning electron microscopy data showed that the scaffold was filled with grown chondrocytes and their produced extracellular matrix after 21 days. In addition, glycosaminoglycan (GAG) accumulation in the scaffold culture was at a higher level than the monolayer culture. Cultured cartilage in vitro for 14 days showed enough elasticity and stiffness to be handled in vivo. An articular cartilage defect was initiated in the patellar groove of the femur of rabbits and was subsequently filled with the chondrocyte-cultured ACHMS scaffold, ACHMS scaffold alone, or nonfilled (control). Three months after the operations, histological analysis showed that only defects inserted with chondrocytes being cultured in ACHMS scaffolds were filled with reparative hyaline cartilage, and thereby highly expressing type II collagen. These results indicate that implantation of allogenic chondrocytes cultured in ACHMS scaffolds may be effective in repairing articular cartilage defects. (c) 2005 Wiley Periodicals, Inc.