Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division
Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division
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DOI：
10.1039/d0nr00242a
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发表时间：
2020-06-14
期刊：
影响因子：
6.7
通讯作者：
Doi, Nobuhide
中科院分区：
文献类型：
--
作者：
Kohyama, Shunshi;Fujiwara, Kei;Doi, Nobuhide
用于确定细菌中心细胞分裂位置的 Min 系统具有独特的特征，它使用从其组件（MinD 和 MinE）中出现的蛋白质波（Min 波）。 Min波是在MinDE的化学反应和分子扩散的耦合下出现的，并且当MinD和MinE的浓度相似时出现。然而，确定其浓度范围的纳米级机制仍然难以捉摸。在这项研究中，通过使用人造细胞作为细胞的模拟物，我们发现主要的 MinE 构象决定了 Min 波出现的允许浓度范围。此外，MinE 膜结合区域的缺失表明该区域对于限制浓度范围更窄至关重要。这些发现说明了纳米尺度复杂分子系统中用于活细胞时空调节的参数调节机制，并表明分子构象之间平衡的调节可以作为细胞分裂的开关。
The Min system for determining the cell division position at the center in bacteria has a unique character that uses a protein wave (Min wave) that emerges from its components (MinD and MinE). The Min wave emerges under the coupling of chemical reactions and molecular diffusions of MinDE and appears when the concentrations of MinD and MinE are similar. However, the nanoscale mechanism to determine their concentration ranges has remained elusive. In this study, by using artificial cells as a mimic of cells, we showed that the dominant MinE conformations determined the allowable concentration ranges for the emergence of the Min wave. Furthermore, the deletion of the membrane-binding region of MinE indicated that the region was essential for limiting the concentration ranges to be narrower. These findings illustrate a parameter tuning mechanism underlying complex molecular systems at the nanoscale for spatiotemporal regulation in living cells and show a possibility that the regulation of the equilibrium among molecular conformations can work as a switch for cell division.