Differential expression of proteins in maize roots in response to abscisic acid and drought
Differential expression of proteins in maize roots in response to abscisic acid and drought
复制标题
玉米根部蛋白质响应脱落酸和干旱的差异表达
DOI：
10.1007/s11738-011-0784-y
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发表时间：
2011-11-01
影响因子：
2.6
通讯作者：
Zhang, Jie
中科院分区：
文献类型：
--
作者：
Hu, Xiuli;Lu, Minghui;Zhang, Jie
根是植物对干旱反应高度敏感的器官，通常会抑制根的生长。然而，关于ABA对干旱诱导的根蛋白表达的影响，人们知之甚少。为了阐明ABA在根对干旱反应的蛋白质表达中的作用，利用蛋白质组学方法对干旱胁迫下玉米ABA缺失突变体Vp5及其野生型Vp5的根系蛋白质谱进行了研究。利用双向电泳法对玉米根系中的干旱响应蛋白斑点进行鉴定。考马斯亮蓝染色后，在每个凝胶上重复检测到约450个蛋白质点，其中22个与ABA或干旱相关的蛋白质点被MALDI-TOF MS鉴定。结果表明，这22个蛋白质参与了能量和代谢、氧化还原动态平衡和调节等几个细胞过程。干旱以ABA依赖的方式上调阴离子过氧化物酶和两个可能的未知蛋白，干旱以ABA依赖和非ABA依赖的方式上调富含甘氨酸的RNA结合蛋白2、病程相关蛋白10、烯醇化酶、丝氨酸/苏氨酸蛋白激酶受体和胞质抗坏血酸过氧化物酶，干旱以ABA不依赖的方式上调核运输因子2、核苷二磷酸激酶、未知蛋白和过氧化还原蛋白-5；干旱对超氧化物歧化酶4A、VAP27-2、转录因子BTF3、谷胱甘肽S转移酶GSTF2和一个未知蛋白的表达有上调作用，但外源ABA在无干旱条件下不表达；AO-甲基转移酶和未知蛋白受ABA和干旱共同下调。一些新的干旱响应蛋白的发现为更好地理解根的耐旱性的分子基础提供了新的见解。
Roots are highly sensitive organ in plant response to drought, which commonly inhibits root growth. However, less is known about the effect of ABA on root protein expression induced by drought. To help clarify the role of ABA in protein expression of root response to drought, root protein patterns were monitored using a proteomic approach in maize ABA-deficient mutantvp5and its wild-typeVp5exposed to drought. Two-dimensional electrophoresis was used to identify drought-responsive protein spots in maize roots. After coomassie brilliant blue staining, approximately 450 protein spots were reproducibly detected on each gel, wherein 22 protein spots related to ABA or drought were identified using MALDI-TOF MS. Results showed that the 22 proteins are involved in such several cellular processes as energy and metabolism, redox homeostasis and regulatory. An anionic peroxidase and two putative uncharacterized proteins were up-regulated by drought in ABA-dependent way; A glycine-rich RNA binding protein 2, pathogenesis-related protein 10, an enolase, a serine/threonine-protein kinase receptor and a cytosolic ascorbate peroxidase were up-regulated by drought in both ABA-dependent and ABA-independent way; a nuclear transport factor 2, a nucleoside diphosphate kinase, a putative uncharacterized protein and a peroxiredoxin-5 were up-regulated by drought in ABA-independent way; a superoxide dismutase 4A, a VAP27-2, a transcription factor BTF3, a glutathione S-transferase GSTF2 and a putative uncharacterized protein were up-regulated by drought in ABA-dependent way, but not exogenous ABA treatment in the absence of drought; aO-methyltransferase and a putative uncharacterized proteins were down-regulated by ABA and drought. The identification of some novel proteins in the drought response provides new insights that can lead to a better understanding of the molecular basis of root drought tolerance.