A Set of Standard Differential Blast Isolates (Magnaporthe grisea (Hebert) Barr.) from the Philippines for Rice (Oryza sativa L.) Resistance
A Set of Standard Differential Blast Isolates (Magnaporthe grisea (Hebert) Barr.) from the Philippines for Rice (Oryza sativa L.) Resistance
复制标题
一组来自菲律宾的标准差异稻瘟病分离株（Magnaporthe grisea (Hebert) Barr.）对水稻 (Oryza sativa L.) 的抗性
DOI：
10.6090/jarq.42.23
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发表时间：
2008
影响因子：
0.4
通讯作者：
Y. Fukuta
中科院分区：
文献类型：
--
作者：
Mary Jeanie Telebanco;T. Imbe;H. Kato;H. Tsunematsu;L. A. Ebron;C. Cruz;N. Kobayashi;Y. Fukuta
共检测到119株稻瘟病菌（Magnaporthe grisea（Hebert）巴尔.）从菲律宾收集的分离物，其致病性进行了表征，用19个水稻（Oryza sativa L.）针对18个抗病基因的差异品种（DV）。首先，根据针对9个抗性基因Pia、Pib、Pii、Pit、Pita、Pish、Piz-t、Pi 3和Piz-5的9个DV的反应模式，将这些分离物分为31个组，然后考虑其他9个DV对其他抗性基因Pik、Pik-h、Pik-m、Pik-p、Pik-s、Pita-2、Piz、Pil的反应模式，将这些分离物进一步分为70个致病型，Pi20（t）。筛选出20个具有分化能力、反应稳定、产孢能力强的菌株。使用单基因系作为靶向24个抗性基因Pia、Pib、Pii、Pik、Pik-h、Pik-m、Pik-p、Pik-s、Pish、Pit、Pita、Pita-2、Piz、Piz-t、Piz-5、Pil、Pi 3、Pi 5（t）、Pi 7（t）、Pi 9（t）、Pil（t）、Pi 2（t）、Pi 19（t）和Pi 20（t）的DV组来确认这些分离株的反应。Pik、Pik-h、Pik-m、Pik-p和Pil的几个抗性等位基因具有相同的反应模式，不能被这些选择的稻瘟病菌株区分。未发现Pit和Pil 9的无毒菌株，Pish、Piz-5和Piz三个基因对这两个菌株表现出广泛的中等抗性。这些研究结果表明，在菲律宾存在着各种各样的爆炸病原体。菲律宾稻瘟病菌株致病性的信息也将有助于了解稻瘟病小种和抗性基因之间的分化和关系。以单基因系为DVs，选取20个稻瘟病菌菌株作为一级鉴别体系，可用于鉴定热带地区水稻品种的抗病性和稻瘟病菌菌株的致病性。
A total of 119 blast (Magnaporthe grisea (Hebert) Barr.) isolates collected from the Philippines, were characterized for their pathogenicities using 19 rice (Oryza sativa L.) differential varieties (DVs) targeting for 18 resistance genes. These isolates were classified into 31 groups based on the reaction patterns to 9 DVs for targeting 9 resistance genes, Pia, Pib, Pii, Pit, Pita, Pish, Piz-t, Pi3, and Piz-5, at first, and then further divided into 70 pathotypes considering the reaction pattern of the other 9 DVs for the other resistance genes, Pik, Pik-h, Pik-m, Pik-p, Pik-s, Pita-2, Piz, Pil, and Pi20(t). Twenty isolates that have differentiating ability, stable reactions and good sporulating ability were selected. The reactions of these isolates were confirmed using the monogenic lines as a set of DVs for targeting 24 resistance genes, Pia, Pib, Pii, Pik, Pik-h, Pik-m, Pik-p,Pik-s, Pish, Pit, Pita, Pita-2, Piz, Piz-t, Piz-5, Pil, Pi3, Pi5(t), Pi7(t), Pi9(t), Pill(t), Pil2(t), Pi19(t), and Pi20(t). Several resistance alleles of Pik locus, Pik, Pik-h, Pik-m, and Pik-p, and Pil had the same reaction patterns and could not be differentiated by these selected blast isolates. No avirulent isolate for Pit and Pil9 was found. The three genes, Pish, Piz-5, and Piz, showed a wide spectrum of moderate resistances to these isolates. These findings suggested the existence of a wide variation of blast pathogens in the Philippines. This information on pathogenicity of blast isolates from the Philippines will be also useful to understand the differentiation and relationship between blast races and resistance genes. The monogenic lines as the DVs and the selected 20 blast isolates can be used as the first differential system, which can characterize the resistances of rice varieties and pathogenicities of blast isolates in the tropics.