A study of the role of gene TaMYB2 and an associated SNP in dehydration tolerance in common wheat

A study of the role of gene TaMYB2 and an associated SNP in dehydration tolerance in common wheat
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DOI:
10.1007/s11033-012-1983-3
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发表时间:
2012-12-01
影响因子:
2.8
通讯作者:
Prasad, Manoj
Prasad, Manoj
中科院分区:
生物学4区
文献类型:
--
作者:
Garg, Bharti;Lata, Charu;Prasad, Manoj

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成髓细胞瘤癌基因(myeloblastosis oncogene,MYB)是一种重要的转录因子,参与多种发育和应激反应基因的诱导。因此,它们正在成为提高植物对几种非生物胁迫的胁迫耐受性的关键参与者。因此,这些基因的分离和鉴定、有用等位基因的转基因和功能分子标记的开发是各种作物改良计划的核心。在本论文中,我们首次报道了小麦TaMYB 2基因第458 bp(A/G转换)处与脱水耐受性相关的同义单核苷酸多态性的鉴定。以及开发用于其脱水耐受性的等位基因特异性标记(ASM)。此外,我们在28个小麦品种的核心组中验证了该TaMYB 2-ASM,其可用于植物育种计划中脱水耐性的标记辅助选择。
The myeloblastosis oncogenes (MYB) are one of the important transcription factors that facilitate induction of various developmental and stress responsive genes. They are hence, emerging as key players in improving stress tolerance of plants in response to several abiotic stresses. Therefore, isolation and characterization of these genes, development of transgenics and functional molecular markers for useful alleles is central to various crop improvement programs. In this manuscript, we for the first time are reporting the identification of a synonymous single nucleotide polymorphism associated with dehydration tolerance at 458th bp (an A/G transition) in the TaMYB2 gene of wheat (Triticum aestivum L.) and development of an allele-specific marker (ASM) for dehydration tolerance for the same. Further we validated this TaMYB2-ASM in a core set of 28 wheat cultivars which can be used for marker-assisted selection for dehydration tolerance in plant breeding programs.