A COLORIMETRIC METHOD FOR THE DETERMINATION OF SERUM GLUTAMIC OXALACETIC AND GLUTAMIC PYRUVIC TRANSAMINASES
A COLORIMETRIC METHOD FOR THE DETERMINATION OF SERUM GLUTAMIC OXALACETIC AND GLUTAMIC PYRUVIC TRANSAMINASES
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DOI：
10.1093/ajcp/28.1.56
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发表时间：
1957-01-01
影响因子：
3.5
通讯作者：
FRANKEL, S
中科院分区：
文献类型：
--
作者：
REITMAN, S;FRANKEL, S
结果用分光光度法和比色法测定血清中谷草转氨酶和谷丙转氨酶的活性。所有的比色测定都是用博士伦色度计进行的，使用的是15 mm。小甜心。用DU Beckman分光光度计进行分光光度测定，并将结果校正至25℃。6将光密度的变化与转氨酶活性绘制成图，按Karmen2定义的单位表示(校正至25℃)，并以最佳直线拟合点(图2和图3)。表1中的数据是对121份血清和34份血清进行谷草转氨酶(GOT)活性和谷丙转氨酶(GPT)活性测定结果的比较。相关系数分别为0.97和0.91。
RESULTSSerums were assayed for activity of GOT and GPT by the spectrophotometry and by the colorimetric procedures. All of the colorimetric determinations were made with a Bausch and Lomb colorimeter, using a 15-mm. cuvet. The spectrophotometric determinations were made with a DU Beckman spectrophotometer and the results were corrected to 25 C. 6 The change in optical density was plotted against the activity of transaminase, expressed in units as defined by Karmen2 (corrected to 25 C), and the best line fitted to the points (Figs. 2 and 3). The data in Table 1 represent a comparison of the results obtained from the assay of 121 serums for the activity of GOT and 34 serums for the activity of GPT. The correlation coefficients were, respectively, 0.97 and 0.91.