Common-path interferometric detection of protein monolayer on the BioCD
Common-path interferometric detection of protein monolayer on the BioCD
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DOI：
10.1364/ao.46.007836
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发表时间：
2007-11-10
期刊：
影响因子：
1.9
通讯作者：
Nolte, D. D.
中科院分区：
文献类型：
--
作者：
Wang, Xuefeng;Zhao, Ming;Nolte, D. D.
生物光学光盘(BioCD)是一种光学生物传感器，可以对高速旋转的光盘上的图案化蛋白质进行共路分子干涉测量。共光路配置使其超稳定，并允许地面高度精度低于10 pm在本文中，我们证明了两个互补的干涉正交条件同时存在，它将由盘表面蛋白质图案调制的反射系数的模数和相位转换为探测器处的强度调制。在远场，它们根据蛋白质的局部分布分为空间对称强度调制和反对称强度调制。反对称响应等效于差分相位衬度检测，对称响应等效于同轴(IL)共路干涉测量。我们测量了这些正交通道对盘结构上的印刷蛋白质图案的相对灵敏度，其中包括硅上的热氧化物和布拉格电介质堆栈。测得硅上氧化物离子通道的标度质量灵敏度为0.17pg/mm。(C)2007年美国光学学会。
The bio-optical compact disk (BioCD) is an optical biosensor that performs common-path molecular interferometry of patterned proteins on a disk spinning at high speed. The common-path configuration makes it ultrastable and allows surface height precision below 10 pm. In this paper we show that two complementary interferometric quadrature conditions exist simultaneously that convert the modulus and phase of the reflection coefficient, modulated by protein patterns on the disk surface, into intensity modulation at the detector. In the far field they separate into spatially symmetric and antisymmetric intensity modulation in response to the local distribution of protein. The antisymmetric response is equivalent to differential phase-contrast detection, and the symmetric response is equivalent to in-line (IL) common-path interferometry. We measure the relative sensitivities of these orthogonal channels to printed protein patterns on disk structures that include thermal oxide on silicon and Bragg dielectric stacks. The scaling mass sensitivity of the IL channel on oxide on silicon was measured to be 0.17 pg/mm. (C) 2007 Optical Society of America.