New Insights on Leucine-Rich Repeats Receptor-Like Kinase Orthologous Relationships in Angiosperms.
New Insights on Leucine-Rich Repeats Receptor-Like Kinase Orthologous Relationships in Angiosperms.
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DOI：
10.3389/fpls.2017.00381
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发表时间：
2017
影响因子：
5.6
通讯作者：
Diévart A
中科院分区：
文献类型：
--
作者：
Dufayard JF;Bettembourg M;Fischer I;Droc G;Guiderdoni E;Périn C;Chantret N;Diévart A
富含亮氨酸重复序列的受体样激酶（LRR-RLK）基因是植物中一个庞大而复杂的基因家族，主要参与植物的发育和逆境应答。这些受体由含LRR的胞外结构域（ECD）、跨膜结构域（TM）和胞内激酶结构域（KD）组成。为了对这些基因在模式和非模式植物物种中的功能分析提供新的视角，我们对31种被子植物基因组（8种单子叶植物和23种双子叶植物）中的8，360个LRR-RLK受体进行了系统发育分析。我们确定了101个正交基因组（OG）的基因几乎所有的单子叶植物和双子叶植物物种中的保守分析。我们观察到，超过10%的这些OG是缺席的，在所研究的菊科物种。我们发现，ECD的结构特征并不总是保守的直系同源物之间，这表明功能可能有分歧，在一些OG集。此外，我们观察了12对OG中的正选择足迹的目标，并注意到根据亚组，正选择在ECD或KD中更频繁地发生。
Leucine-Rich Repeats Receptor-Like Kinase (LRR-RLK) genes represent a large and complex gene family in plants, mainly involved in development and stress responses. These receptors are composed of an LRR-containing extracellular domain (ECD), a transmembrane domain (TM) and an intracellular kinase domain (KD). To provide new perspectives on functional analyses of these genes in model and non-model plant species, we performed a phylogenetic analysis on 8,360 LRR-RLK receptors in 31 angiosperm genomes (8 monocots and 23 dicots). We identified 101 orthologous groups (OGs) of genes being conserved among almost all monocot and dicot species analyzed. We observed that more than 10% of these OGs are absent in the Brassicaceae species studied. We show that the ECD structural features are not always conserved among orthologs, suggesting that functions may have diverged in some OG sets. Moreover, we looked at targets of positive selection footprints in 12 pairs of OGs and noticed that depending on the subgroups, positive selection occurred more frequently either in the ECDs or in the KDs.