Use of meso-scale discovery to examine cytokine content in microglia cell supernatant.
Use of meso-scale discovery to examine cytokine content in microglia cell supernatant.
复制标题
DOI：
10.1007/978-1-62703-520-0_11
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Burguillos, Miguel A
中科院分区：
文献类型：
--
作者：
Burguillos, Miguel A
由活化的小胶质细胞产生的细胞因子是CNS中炎症反应的标志之一。由小胶质细胞释放的细胞因子可以根据促炎刺激而非常不同。传统上，为了定量这些不同的细胞因子，已经使用“三明治”-酶联免疫吸附测定（三明治-ELISA）。在本章中，我们将讨论和描述一个改进的方案的夹心ELISA开发的Meso-Scale Discovery的基础上的电化学发光检测系统，它允许超低检测多种细胞因子的小胶质细胞上清液。
Cytokine production by activated microglia is one of the hallmarks of inflammatory response in the CNS. The cytokines released by microglia cells can be very different depending on the proinflammatory stimulus. Traditionally, to quantify these different cytokines, the "Sandwich"-enzyme-linked immunosorbent assay (Sandwich-ELISA) has been used. In this chapter we will discuss and describe an improved protocol of the Sandwich-ELISA developed by Meso-Scale Discovery based on an electrochemiluminescence detection system, which allows the ultralow detection of multiple cytokines in microglia cell supernatant.