Survey and Characterization of Jingmen Tick Virus Variants
Survey and Characterization of Jingmen Tick Virus Variants
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DOI：
10.3390/v11111071
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发表时间：
2019-11-01
期刊：
影响因子：
4.7
通讯作者：
Ergunay, Koray
中科院分区：
文献类型：
--
作者：
Dincer, Ender;Hacioglu, Sabri;Ergunay, Koray
我们获得了一个荆门蜱病毒（JMTV）分离物，接种蜱池与可检测的克里米亚-刚果出血热病毒（CCHFV）RNA。随后，我们筛选了7223蜱，代表15种，在5个属，收集来自不同地区的安纳托利亚和色雷斯东部，土耳其。此外，我们检测了来自不同患者队列（n = 103）的标本，以及犬（n = 60）、牛（n = 20）和禽类标本（n = 65）。在3.9%的蜱池中检测到JMTV核酸，包括来自扇头蜱属和血蜱属的几个蜱种以及土耳其CCHFV的主要载体Hyalomma marginatum的蜱。系统发育分析支持两个独立的分支，独立的主机或位置，这表明普遍存在的分布在蜱。JMTV未从检测的任何人、动物或鸟类标本中回收。从原型分离株和三个感染蜱池中对接近完整的病毒基因组进行测序。基因组拓扑结构和功能组织与荆门组病毒成员一致。单个病毒基因组片段和功能元件的系统发育重建进一步支持来自科索沃的菌株的密切关系。我们进一步确定了JMTV基因组中可能的重组事件，涉及来自安纳托利亚或中国的密切相关的菌株。
We obtained a Jingmen tick virus (JMTV) isolate, following inoculation of a tick pool with detectable Crimean-Congo hemorrhagic fever virus (CCHFV) RNA. We subsequently screened 7223 ticks, representing 15 species in five genera, collected from various regions in Anatolia and eastern Thrace, Turkey. Moreover, we tested specimens from various patient cohorts (n = 103), and canine (n = 60), bovine (n = 20) and avian specimens (n = 65). JMTV nucleic acids were detected in 3.9% of the tick pools, including those from several tick species from the genera Rhipicephalus and Haemaphysalis, and Hyalomma marginatum, the main vector of CCHFV in Turkey. Phylogenetic analysis supported two separate clades, independent of host or location, suggesting ubiquitous distribution in ticks. JMTV was not recovered from any human, animal or bird specimens tested. Near-complete viral genomes were sequenced from the prototype isolate and from three infected tick pools. Genome topology and functional organization were identical to the members of Jingmen group viruses. Phylogenetic reconstruction of individual viral genome segments and functional elements further supported the close relationship of the strains from Kosovo. We further identified probable recombination events in the JMTV genome, involving closely-related strains from Anatolia or China.