Unusually long mRNA species coding for human alpha and beta interferons.
Unusually long mRNA species coding for human alpha and beta interferons.
复制标题
编码人类 α 和 β 干扰素的异常长 mRNA 种类。
DOI：
10.1073/pnas.79.22.6932
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发表时间：
1982
影响因子：
11.1
通讯作者：
Inouye,M
中科院分区：
文献类型：
--
作者：
Sehgal,PB;May,LT;LaForge,KS;Inouye,M
几个无内含子干扰素(IFN) α S基因中的每一个都嵌入在4到5千碱基（kb）的DNA同源单位中，这些同源单位在人类基因组中或多或少地串联排列。除了预期的0.8- 1.4 kb长的ifn - α - S mRNA物种外，我们还在仙台病毒诱导的溴脱氧尿嘧啶处理的人淋巴母细胞（Namalwa）细胞质中检测到大约2-2.7、3.5-4.5和7-8 kb长的ifn - α - S基因的多腺苷化RNA分子。这些转录本通过琼脂糖- ch3hgoh凝胶对细胞质多腺苷化RNA进行电泳检测，然后使用IFN- α 1（原型α S） cDNA探针进行blothybrization，并在非洲爪蟾卵母细胞试验中将洗脱的RNA翻译成具有生物活性的IFN。至少这些长翻译活性RNA分子中的一些似乎代表了功能性mRNA物种，因为它们可以在多聚体中检测到。此外，卵母细胞翻译实验显示，在仙台病毒诱导的Namalwa细胞和poly(I)中都存在异常长的（2-8 kb） ifn - β mRNA物种。聚(C)诱导的二倍体人成纤维细胞（FS-4）。超长ifn - α 1相关mRNA物种的检测表明，在侧翼同源单位中观察到的一些DNA序列特征可能影响ifn - α S基因的转录。我们的杂交分析还表明，1.8 kb的人类ifn - α L mRNA(s)与ifn - α 1 cDNA探针没有可检测到的交叉杂交。因此，这种mRNA来源于一个不同于ifn - α - s组的基因。
Each of the several intronless interferon (IFN) alpha S genes is embedded in 4- to 5-kilobase (kb) DNA homology units which are arranged more or less in tandem in the human genome. In addition to the expected 0.8- to 1.4-kb-long IFN-alpha S mRNA species, we have detected polyadenylylated RNA molecules approximately 2-2.7, 3.5-4.5, and 7-8 kb long derived from IFN-alpha S genes in the cytoplasm of Sendai virus-induced bromodeoxyuridine-treated human lymphoblastoid (Namalwa) cells. These transcripts were detected by electrophoresis of cytoplasmic polyadenylylated RNA through agarose-CH3HgOH gels followed by blothybridization using an IFN-alpha 1 (a prototype alpha S) cDNA probe and also by translation of the eluted RNA into biologically active IFN in the Xenopus oocyte assay. At least some of these long translationally active RNA molecules appear to represent functional mRNA species because they can be detected in polysomes. In addition, the oocyte translation assay has revealed the presence of unusually long (2-8 kb) IFN-beta mRNA species both in Sendai virus-induced Namalwa cells and in poly(I) . poly(C)-induced diploid human fibroblasts (FS-4). The detection of very long IFN-alpha 1-related mRNA species suggests that some of the DNA sequence features observed in the flanking homology units may affect the transcription of the IFN-alpha S genes. Our hybridization analyses also indicate that the 1.8-kb human IFN-alpha L mRNA(s) shows no detectable cross-hybridization with an IFN-alpha 1 cDNA probe. Thus, this mRNA is derived from a gene(s) that is distinct from the IFN-alpha S set.