Determination of bacterial rod shape by a novel cytoskeletal membrane protein
Determination of bacterial rod shape by a novel cytoskeletal membrane protein
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DOI：
10.1038/emboj.2008.234
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发表时间：
2008-12-03
期刊：
影响因子：
11.4
通讯作者：
Niki, Hironori
中科院分区：
文献类型：
--
作者：
Shiomi, Daisuke;Sakai, Masako;Niki, Hironori
细胞形状对生长至关重要，一些基因参与细菌细胞形态发生。在这里，我们报告了一个新的基因，rodZ，需要在大肠杆菌中的杆形状的测定。缺少rodZ的细胞不再具有杆状，而是圆形或椭圆形。这些圆形细胞比已知的圆形突变细胞小，包括mreB和pbpA突变体;两者都已知失去杆状。形态发生从杆细胞到圆细胞，反之亦然，所造成的耗竭和过度生产的RodZ，分别揭示，RodZ可以调节细胞的长轴的长度。RodZ是具有双位拓扑结构的膜蛋白，使得包括螺旋-转角-螺旋基序的N-末端区域在细胞质中，而C-末端区域暴露在周质中。GFP-RodZ在细胞膜下沿细胞横轴沿着形成螺旋，类似于MreB细菌肌动蛋白。因此，RodZ可能介导的空间信息从细胞质中的细胞骨架蛋白质的肽聚糖合成机制的周质。
Cell shape is critical for growth, and some genes are involved in bacterial cell morphogenesis. Here, we report a novel gene, rodZ, required for the determination of rod shape in Escherichia coli. Cells lacking rodZ no longer had rod shape but rather were round or oval. These round cells were smaller than known round mutant cells, including mreB and pbpA mutants; both are known to lose rod shape. Morphogenesis from rod cells to round cells and vice versa, caused by depletion and overproduction of RodZ, respectively, revealed that RodZ could regulate the length of the long axis of the cell. RodZ is a membrane protein with bitopic topology such that the N-terminal region including a helix-turn-helix motif is in the cytoplasm, whereas the C-terminal region is exposed in the periplasm. GFP-RodZ forms spirals along the lateral axis of the cell beneath the cell membrane, similar to the MreB bacterial actin. Thus, RodZ may mediate spatial information from cytoskeletal proteins in the cytoplasm to a peptidoglycan synthesis machinery in the periplasm.