Polymeric nanoparticulate delivery system for Indocyanine green: Biodistribution in healthy mice
Polymeric nanoparticulate delivery system for Indocyanine green: Biodistribution in healthy mice
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DOI：
10.1016/j.ijpharm.2005.11.003
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发表时间：
2006-02-03
影响因子：
5.8
通讯作者：
Shao, J
中科院分区：
文献类型：
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作者：
Saxena, V;Sadoqi, M;Shao, J
本研究的目的是研究通过聚合物纳米粒子递送吲哚菁绿 (ICG) 在健康小鼠体内的生物分布。对包埋 ICG 的聚（DL-乳酸-乙醇酸）（PLGA）纳米颗粒进行了设计和表征。开发了从生物样品中回收ICG的提取方法。使用荧光测定方法，通过 PLGA 纳米粒子递送健康 C57BL/6 小鼠（雌性，10 周龄），与游离 ICG 溶液进行比较，确定 ICG 的生物分布。 ICG 的提取 9 方法对各种器官和血浆显示效率超过 80%。当使用纳米颗粒递送 ICG 时，在静脉注射后 4 小时内，各器官中沉积的 ICG 浓度高出 2-8 倍，血浆水平高出 5-10 倍。与游离 ICG 溶液相比的剂量。总之，纳米颗粒制剂显着增加了ICG在血浆中的浓度和循环时间以及ICG在各个器官中的摄取、积累和保留。总的来说，这项研究代表了探索和建立纳米粒子作为 ICG 递送系统用于肿瘤诊断和光动力治疗的潜力的第一步。 (c) 2005 Elsevier B.V. 保留所有权利。
The objective of this study is to investigate the biodistribution of Indocyanine green (ICG) in healthy mice, when delivered through polymeric nanoparticles. The poly(DL-lactic-co-glycolic acid) (PLGA) nanoparticles entrapping ICG were engineered and characterized. The extraction method for ICG recovery from biological samples was developed. The biodistribution of ICG was determined in healthy C57BL/6 mice (female, 10-week old) when delivered through PLGA nanoparticles in comparison to free ICG solution, using a fluorometric assay method. The extraction 9 method for ICG shows efficiency above 80% for various organs and plasma. When nanoparticles were used to deliver ICG, 2-8 times higher concentrations of ICG was deposited in various organs, with 5-10 times higher plasma levels till 4 h, after an i.v. dose as compared to free ICG solution. In conclusion, the nanoparticle formulation significantly increased the ICG concentration and circulation time in plasma as well as the ICG uptake, accumulation and retention in various organs. Overall, this study represents the first step in exploring and establishing the potential of nanoparticles as an ICG-delivery system for use in tumor-diagnosis and photodynamic therapy. (c) 2005 Elsevier B.V. All rights reserved.