Anillin and the septins promote asymmetric ingression of the cytokinetic furrow
Anillin and the septins promote asymmetric ingression of the cytokinetic furrow
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DOI：
10.1016/j.devcel.2007.02.018
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发表时间：
2007-05-01
影响因子：
11.8
通讯作者：
Oegema, Karen
中科院分区：
文献类型：
--
作者：
Maddox, Amy Shaub;Lewellyn, Lindsay;Oegema, Karen
在胞质分裂过程中，皮层收缩环的收缩产生了一个沟，将一个细胞分成两个。收缩环包含三个纤维系统：肌动蛋白，双极肌球蛋白II丝，和隔蛋白，GTP结合的异源寡聚体，形成一个膜相关的晶格。收缩环还含有潜在的细丝交联剂，苯胺，其结合所有三种细丝类型。在这里，我们表明，收缩环具有内在的破膜机制，促进不对称开沟。不对称的内陷需要苯胺和septins，它们促进收缩环一侧的组分聚结，但对肌球蛋白11水平降低10倍不敏感。当不对称性被破坏时，胞质分裂对收缩性的部分抑制变得敏感。因此，不对称沟内移，一个普遍的，但以前未探索的功能，在后生动物细胞分裂，是由两个保守的沟组件的行动产生的，并担任机械功能，使胞质分裂鲁棒。
During cytokinesis, constriction of a cortical contractile ring generates a furrow that partitions one cell into two. The contractile ring contains three filament systems: actin, bipolar myosin II filaments, and septins, GTP-binding hetero-oligomers that polymerize to form a membrane-associated lattice. The contractile ring also contains a potential filament crosslinker, Anillin, that binds all three filament types. Here, we show that the contractile ring possesses an intrinsic symmetry-breaking mechanism that promotes asymmetric furrowing. Asymmetric ingression requires Anillin and the septins, which promote the coalescence of components on one side of the contractile ring, but is insensitive to a 10-fold reduction in myosin 11 levels. When asymmetry is disrupted, cytokinesis becomes sensitive to partial inhibition of contractility. Thus, asymmetric furrow ingression, a prevalent but previously unexplored feature of cell division in metazoans, is generated by the action of two conserved furrow components and serves a mechanical function that makes cytokinesis robust.