INFECTIOUS RNA TRANSCRIBED FROM STABLY CLONED FULL-LENGTH CDNA OF DENGUE TYPE-4 VIRUS
INFECTIOUS RNA TRANSCRIBED FROM STABLY CLONED FULL-LENGTH CDNA OF DENGUE TYPE-4 VIRUS
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DOI：
10.1073/pnas.88.12.5139
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发表时间：
1991-06-01
影响因子：
11.1
通讯作者：
BRAY, M
中科院分区：
文献类型：
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作者：
LAI, CJ;ZHAO, B;BRAY, M
登革病毒是一种有包膜的正链RNA病毒，其基因组长度几乎等于11个内切酶。登革热病毒的四种血清型目前在地理分布和人类感染率方面是黄病毒家族中最重要的成员。在这篇文章中，我们描述了成功克隆登革4型病毒的一个稳定的全长cDNA拷贝，该拷贝可用作感染性RNA体外转录的模板。有证据表明，从体外RNA转录物转染的许可细胞中回收的登革病毒保留了一个突变，该突变被改造成全长cDNA。用登革cDNA的感染性RNA转录物转染的细胞产生的病毒的性质类似于cDNA克隆所来源的病毒的性质。登革病毒重组DNA系统应证明有助于更好地了解登革病毒的分子生物学，并应促进开发用于人类的安全有效的活疫苗。
Dengue virus is an enveloped positive-strand RNA virus with a genome almost-equal-to 11 kilobases in length. The four serotypes of dengue virus are currently the most important members of the flavivirus family in terms of geographical distribution and the incidence of infection in humans. In this communication we describe successful cloning of a stable full-length cDNA copy of dengue type 4 virus that can be used as the template for in vitro transcription of infectious RNA. Evidence is presented that dengue virus recovered from permissive cells transfected with the in vitro RNA transcripts retained a mutation that was engineered into full-length cDNA. The properties of the virus produced by cells transfected with infectious RNA transcripts of dengue cDNA resembled those of the virus from which the cDNA clone was derived. The dengue virus recombinant DNA system should prove helpful in gaining a better understanding of the molecular biology of dengue viruses and should facilitate the development of a safe and effective live vaccine for use in humans.