Improvement of microvascular endothelial dysfunction induced by exercise and diet is associated with microRNA-126 in obese adolescents
Improvement of microvascular endothelial dysfunction induced by exercise and diet is associated with microRNA-126 in obese adolescents
复制标题
肥胖青少年运动和饮食引起的微血管内皮功能障碍的改善与 microRNA-126 相关
DOI：
10.1016/j.mvr.2018.10.009
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发表时间：
2019-05-01
影响因子：
3.1
通讯作者：
Yang Dongsheng
中科院分区：
文献类型：
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作者：
Tang Donghui;Bai Shuang;Yang Dongsheng
目的：微血管内皮功能障碍先于大血管内皮功能障碍，处于动脉粥样硬化的早期阶段。本研究旨在探讨运动和饮食改善肥胖青少年微血管内皮功能障碍的机制。（57名肥胖男性青少年;年龄：15.38 ± 2.82岁，BMI：33.21 ± 4.23 kg/m2）完成了为期6周的饮食干预运动计划，对照组（10名正常体重的青少年;年龄：15.38 +/- 2.82岁，BMI：23.21 +/- 4.23 kg/m（2））保持久坐。治疗6周前后检测患者临床特征、循环NO、ET-1和microRNA-126（miR-126）水平。结果：干预6周后，肥胖青少年的围度和糖脂代谢均得到明显改善。RHI（p < 0.01）和血清NO/ET-1（p < 0.01）水平显著升高，而microRNA-126显著降低（p < 0.01）。Delta MiR-126与Delta BMI正相关（r = 0.60，p < 0.01），Delta RHI（r = 0.69，p < 0.05），和Δ NO/ET-1（r = 0.68，p < 0.05）。运动与饮食控制相结合能有效改善肥胖青少年的糖脂代谢，从而改善其微血管内皮功能，这可能与血清miRNA-126的变化有关。
Objective: Microvascular endothelial dysfunction, which is at the early stage of atherosclerosis, precedes macrovascular endothelial dysfunction. The study is aimed to investigate the mechanism underlying the improvement of microvascular endothelial dysfunction by exercise and diet in obese adolescents.Methods: A quasi-randomized study was carried out with 2 cohorts: the experimental group (57 obese male adolescents; age: 15.38 +/- 2.82 years, BMI: 33.21 +/- 4.23 kg/m(2)) completed a 6-week exercise program with dietary intervention, and control group (10 normal weight adolescents; age: 15.38 +/- 2.82 years, BMI: 23.21 +/- 4.23 kg/m(2)) maintained sedentary. Clinical characteristics, circulating NO, ET-1 and microRNA-126 (miR-126) levels were measured before and after 6 weeks. The Reactive Hyperemia Index (RHI) was measured using EndoPAT-2000 system.Results: After 6-weeks intervention, obese adolescents' body circumferences and glucolipid metabolism are significantly improved. RHI (p < 0.01) and serum levels of NO/ET-1 (p < 0.01) are significantly increased, while microRNA-126 significantly decreased (p < 0.01). Delta MiR-126 were positive correlated with Delta BMI (r = 0.60, p < 0.01), Delta RHI (r = 0.69, p < 0.05), and Delta NO/ET-1 (r = 0.68, p < 0.05).Conclusions: Combination of exercise and diet control can effectively improve glycolipid metabolism of obese adolescents, and thus their microvascular endothelial function, which might be related to changes in serum miRNA-126.