Improvement of (R)-3-hydroxybutyric acid secretion during Halomonas sp. KM-1 cultivation with saccharified Japanese cedar by the addition of urea.
Improvement of (R)-3-hydroxybutyric acid secretion during Halomonas sp. KM-1 cultivation with saccharified Japanese cedar by the addition of urea.
复制标题
盐单胞菌 (Halomonas sp.) 期间 (R)-3-羟基丁酸分泌的改善。
DOI：
10.1111/lam.12473
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
I. Matsushita and J. Tsubota
中科院分区：
文献类型：
--
作者：
Y. Kawata;M. Nojiri;I. Matsushita and J. Tsubota
杉木（Cryptomeria japonica）是日本人工林的主要树种。TheHalomonassp。KM‐1最近被分离出来，并发现在糖化的杉木上有效生长，导致细胞内在有氧条件下储存聚(R)‐3‐羟基丁酸（PHB）。在微氧条件下，细胞外分泌（R)‐3‐羟基丁酸(R)‐3‐HB）导致细胞内PHB的降解。在本研究中，在尿素的存在下，使用糖化杉木的PHB的产生和(R)‐3‐HB的分泌得到了很大的改善。好氧培养36 h后细胞内PHB产量为23·6 g l−1；转到微氧条件24 h后，培养基中的(R)‐3‐HB浓度达到21.1 g l−1。因此，KM‐1有效地利用糖化的杉木生产PHB并分泌(R)‐3‐HB，使其成为工业生产(R)‐3‐HB的实际候选物。研究的意义和影响杉木是日本人工林的主要树种，杉木的疏伐对人工林的健康发展和日本经济至关重要。KM‐1在糖化的杉木上有效生长，导致在有氧条件下细胞内储存聚(R)‐3‐羟基丁酸（PHB）。在微氧条件下，细胞外分泌（R)‐3‐羟基丁酸(R)‐3‐HB）引起细胞内PHB降解。(R)‐3‐HB是一种手性化合物，在化学、保健食品和制药工业中非常有用。利用木质糖化后，PHB的产量和(R)‐3‐HB的分泌量显著提高，这可能对其未来的工业生产产生积极影响。
Japanese cedar (Cryptomeria japonica) is a major species in artificial Japanese forests. TheHalomonassp. KM‐1 was recently isolated and found to grow effectively on saccharified Japanese cedar wood, resulting in the intracellular storage of poly‐(R)‐3‐hydroxybutyric acid (PHB) under aerobic conditions. Under microaerobic conditions, the extracellular secretion of (R)‐3‐hydroxybutyric acid ((R)‐3‐HB) led to the degradation of intracellular PHB. In this study, the production of PHB and the secretion of (R)‐3‐HB using saccharified Japanese cedar were much improved in cultures that were grown in the presence of urea. The level of intracellular PHB production after 36 h under aerobic cultivation was 23·6 g l−1; after shifting to microaerobic conditions for 24 h, the (R)‐3‐HB concentration in the medium reached 21·1 g l−1. Thus, KM‐1 efficiently utilizes saccharified Japanese cedar to produce PHB and secretes (R)‐3‐HB, making it a practical candidate for use in the industrial production of (R)‐3‐HB.Significance and Impact of the StudyJapanese cedar is a major species grown in artificial Japanese forests, and its thinning is crucial for the health of artificial forests and the Japanese economy.Halomonassp. KM‐1 grew effectively on saccharified Japanese cedar wood, resulting in intracellular storage of poly‐(R)‐3‐hydroxybutyric acid (PHB) under aerobic conditions. Under microaerobic conditions, extracellular secretion of (R)‐3‐hydroxybutyric acid ((R)‐3‐HB) caused intracellular PHB degradation. (R)‐3‐HB is a chiral compound that is useful in the chemical, health food and pharmaceutical industries. The production of PHB and secretion of (R)‐3‐HB using saccharified wood was dramatically improved, which may positively affect its future industrial production.