The secretion of a uterine specific, purple phosphatase by cultured explants of porcine endometrium: dependency upon the state of pregnancy of the donor animal.
The secretion of a uterine specific, purple phosphatase by cultured explants of porcine endometrium: dependency upon the state of pregnancy of the donor animal.
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培养的猪子宫内膜外植体分泌子宫特异性紫色磷酸酶：取决于供体动物的妊娠状态。
DOI：
10.1095/biolreprod20.3.431
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发表时间：
1979
影响因子：
3.6
通讯作者：
R. Roberts
中科院分区：
文献类型：
--
作者：
S. M. Mahaboob Basha;F. Bazer;R. Roberts
猪子宫内膜的外植体在体外培养于含有放射性L亮氨酸的改良Eagle‘sMEM4中时，会将标记的蛋白质分泌到生长介质中。在发情周期的第三天，怀孕60天的动物的组织在24小时内向培养液中分泌的放射性物质大约是未怀孕动物的外植体的30倍。怀孕动物的组织分泌物在性质上也是不同的，含有相当数量的两种碱性蛋白，溶菌酶和一种子宫特异性磷酸酶。后者与之前从黄体酮处理过的未怀孕动物的子宫分泌物或尿囊液中提纯的一种含铁紫色蛋白相同。这种蛋白质被认为参与了铁从母亲到胎儿的运输。妊娠30天、45天、60天、75天、90天和105天的动物子宫内膜外植体产生紫色蛋白的能力有相当大的差异。妊娠60天的母猪组织产量最高(2毫克/克组织/24小时)，但妊娠晚期(第105天)的组织产量很低，这一观察结果可能对妊娠母猪的日粮铁补充有相当大的意义。在培养24小时中，孕酮和雌二醇-17a的存在都不会改变外植体产生的蛋白质的数量和质量。我们的结论是，手术时子宫内膜的激素环境主要控制短期培养的外植体蛋白质的数量和质量。
Explants of endometrium from the porcine uterus secrete labeled proteins into the growth medium when incubated in vitro in modified Eagle’s MEM4 containing radioactive L-leucine. Tissue from 60 day pregnant animals secrete about 30 times as much radioactive material into the medium in 24 h as explants taken from nonpregnant animals on Day 3 of the estrous cycle. The secretions by tissue from pregnant animals were also qualitatively distinct and contained appreciable quantities of 2 basic proteins, lysozyme and a uterine-specific phosphatase. The latter was identical to an iron-containing purple protein previously purified either from uterine secretions of progesterone treated, nonpregnant animals or from allantoic fluid. This protein is believed to be involved in iron transport from mother to fetus. Explants from the endometrium of animals on Days 30, 45, 60, 75, 90 and 105 of pregnancy showed considerable differences in their capacities to produce the purple protein. Production was maximal by Day 60 tissue (2 mg/g tissue/24 h), but very low in tissue from late pregnancy (Day 105), an observation which may have considerable implication with regard to dietary iron supplementation of the pregnant sow. Neither the presence of progesterone nor estradiol-17a in the incubation medium altered the quantity or quality of the proteins produced by the explants during the 24 h incubation. We conclude that it is the hormonal environment of the endometrium at the time of surgery which primarily governs the quantity and quality of the proteins by short term cultured explants.