QUANTIFICATION OF HUMAN SERUM PARAOXONASE BY ENZYME-LINKED IMMUNOASSAY - POPULATION DIFFERENCES IN PROTEIN CONCENTRATIONS
QUANTIFICATION OF HUMAN SERUM PARAOXONASE BY ENZYME-LINKED IMMUNOASSAY - POPULATION DIFFERENCES IN PROTEIN CONCENTRATIONS
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DOI：
10.1042/bj3040549
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发表时间：
1994-12-01
影响因子：
4.1
通讯作者：
JAMES, RW
中科院分区：
文献类型：
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作者：
GARIN, MCB;ABBOTT, C;JAMES, RW
对氧磷酶是一种与高密度脂蛋白（HDL）结合的血清蛋白。该酶的生理功能尚不清楚，但已假定在脂质代谢中起作用。迄今为止，蛋白质的研究不得不依赖于对各种底物的酶活性的测量。我们已经开发了一个非常具体的，有竞争力的e.l.i.s.a.使用先前表征的单克隆抗体。该方法可检测20 ng对氧磷酶，工作范围为75-600 ng。批内和批间变异系数分别为6.5%和7.9%。来自日内瓦和曼彻斯特的健康受试者的对氧磷酶血清浓度范围为25至118 μ g/ml。两组之间的平均浓度存在显著差异（日内瓦，79.3+/-18.7 μ g/ml;曼彻斯特，59.9+/-24.1 μ g/ml：P < 0.001），当根据对氧磷酶表型比较受试者时，差异也很明显。这似乎在很大程度上是两个人群之间载脂蛋白A-I浓度差异的结果，表明HDL颗粒数可能在确定血清对氧磷酶水平方面很重要。两组受试者的对氧磷酶比活性也有显著差异（日内瓦，2.08+/-0.96单位/mg;曼彻斯特，3.08+/-1.73单位/mg：P < 0.001），这可能反映了HDL颗粒组成的差异。美国环境解放军。应提供必要的补充对氧磷酶的酶活性的研究，并已提供证据的差异方面的血清水平的蛋白质之间的人口和人口内的表型。
Paraoxonase is a serum protein bound to high-density lipoproteins (HDLs). The physiological function of the enzyme is unknown, but a role in lipid metabolism has been postulated. To date, studies of the protein have had to rely on measurements of enzyme activity with various substrates. We have developed a highly specific, competitive e.l.i.s.a. using a previously characterized monoclonal antibody. The assay can detect 20 ng of paraoxonase with a working range of 75-600 ng. Intra- and interassay coefficients of variation were 6.5 and 7.9% respectively. Serum concentrations of paraoxonase in healthy subjects from Geneva and Manchester ranged from 25 to 118 mu g/ml. There were significant differences in mean concentrations between the two groups (Geneva, 79.3+/-18.7 mu g/ml; Manchester, 59.9+/-24.1 mu g/ml: P < 0.001), differences also apparent when subjects were compared according to paraoxonase phenotype. These appeared to be largely a consequence of differences in apolipoprotein A-I concentrations between the two populations, suggesting that HDL particle number may be important in determining serum levels of paraoxonase. Paraoxonase specific activities were also significantly different between the two groups of subjects (Geneva, 2.08+/-0.96 units/mg; Manchester, 3.08+/-1.73 units/mg: P < 0.001), which may reflect differences in HDL particle composition. The e.l.i.s.a. should furnish the necessary complement to studies of paraoxonase enzymic activity and has already provided evidence for differences with respect to serum levels of the protein both between populations and between phenotypes within populations.