Pseudomonas aeruginosa uses T3SS to inhibit diabetic wound healing.
Pseudomonas aeruginosa uses T3SS to inhibit diabetic wound healing.
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DOI：
10.1111/wrr.12310
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发表时间：
2015-07
期刊：
影响因子：
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通讯作者：
Shafikhani SH
中科院分区：
文献类型：
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作者：
Goldufsky J;Wood SJ;Jayaraman V;Majdobeh O;Chen L;Qin S;Zhang C;DiPietro LA;Shafikhani SH
糖尿病足溃疡比糖尿病的任何其他并发症造成更多的住院治疗。细菌感染被认为是与糖尿病溃疡愈合受损相关的重要因素。铜绿假单胞菌是糖尿病溃疡中最常见的革兰氏阴性病原体。铜绿假单胞菌感染已显示以与其形成生物膜的能力相关的方式损害糖尿病伤口的愈合。虽然糖尿病溃疡中的大多数感染与生物膜相关，但33%的感染本质上是非生物膜。铜绿假单胞菌是所有糖尿病伤口类型中最普遍的革兰氏阴性病原体，这表明铜绿假单胞菌对糖尿病伤口愈合的有害影响超出了其形成生物膜的能力，并且可能涉及其他因素。III型分泌系统（T3SS）毒力结构是所有铜绿假单胞菌临床分离株发病所必需的，这表明它也可能在抑制糖尿病皮肤溃疡的伤口修复中发挥作用。我们评估了T3SS在介导铜绿假单胞菌诱导的糖尿病小鼠伤口组织损伤中的作用。我们的数据表明，铜绿假单胞菌在糖尿病伤口中建立了一种稳健和持续的感染，与其形成生物膜的能力无关，并以主要取决于其T3SS的方式引起严重的伤口损伤。
Diabetic foot ulcers are responsible for more hospitalizations than any other complication of diabetes. Bacterial infection is recognized as an important factor associated with impaired healing in diabetic ulcers. Pseudomonas aeruginosa is the most frequently detected Gram-negative pathogen in diabetic ulcers. P. aeruginosa infection has been shown to impair healing in diabetic wounds in a manner that correlates with its ability to form biofilm. While the majority of infections in diabetic ulcers are biofilm associated, 33% of infections are nonbiofilm in nature. P. aeruginosa is the most prevalent Gram-negative pathogen in all diabetic wound types, which suggests that the deleterious impact of P. aeruginosa on healing in diabetic wounds goes beyond its ability to form biofilm and likely involves other factors. The Type III Secretion System (T3SS) virulence structure is required for the pathogenesis of all P. aeruginosa clinical isolates, suggesting that it may also play a role in the inhibition of wound repair in diabetic skin ulcers. We evaluated the role of T3SS in mediating P. aeruginosa–induced tissue damage in the wounds of diabetic mice. Our data demonstrate that P. aeruginosa establishes a robust and persistent infection in diabetic wounds independent of its ability to form biofilm and causes severe wound damage in a manner that primarily depends on its T3SS.