New structural characteristic of the large glycopeptides from transformed cells
New structural characteristic of the large glycopeptides from transformed cells
复制标题
转化细胞大糖肽的新结构特征
作者：
S. Ogata;T. Muramatsu;A. Kobata
在从正常细胞和转化细胞表面获得的糖肽的比较凝胶过滤中，观察到经病毒、化学和自发方法转化的细胞1-7表面明显大分子的岩藻糖糖肽浓度增加。这被认为是转化表型的表达，因为在被Rous肉瘤病毒温度敏感(Ts)突变体转化的细胞中，重糖肽仅在允许温度下增加。根据唾液酸酶处理的结果，推测额外唾液酸的存在是转化细胞4-6糖肽具有较高分子质量的原因。
IN comparative gel filtration of glycopeptides obtained from the surface of normal and transformed cells, an increased concentration of fucosyl glycopeptides of apparently large molecular weight was observed for cells transformed by viral, chemical and spontaneous methods1–7. This is considered to be an expression of transformed phenotype, since in cells transformed by a temperature-sensitive (ts) mutant of Rous sarcoma virus, the heavy glycopeptides increased only at the permissive temperature. Based on the results of sialidase treatment, it was suggested that the presence of extra sialic acid is responsible for the higher molecular weight nature of the glycopeptides from transformed cells4–6.