In vivo enzymatic protein biotinylation
In vivo enzymatic protein biotinylation
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DOI：
10.1016/s1050-3862(99)00046-7
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发表时间：
1999-12-31
期刊：
影响因子：
--
通讯作者：
Cronan, JE
中科院分区：
文献类型：
--
作者：
Chapman-Smith, A;Cronan, JE
生物素只有与蛋白质结合并共价连接到一类重要的代谢酶，即生物素羧化酶和脱羧酶时才具有生物活性。生物素化是一种相对罕见的修饰，在不同的生物体中发现了一到五种生物素化蛋白。我们讨论了机制和结构参与这种特殊的蛋白质修饰和它在标记重组蛋白的开发。(C) 1999 Elsevier Science B.V.版权所有
Biotin is biologically active only when protein-bound and is covalently attached to a class of important metabolic enzymes, the biotin carboxylases and decarboxylases. Biotinylation is a relatively rare modification, with between one and five biotinylated protein species found in different organisms. We discuss the mechanism and structures involved in this extraordinarily specific protein modification and its exploitation in tagging recombinant proteins. (C) 1999 Published by Elsevier Science B.V. All rights reserved.