THE IMMUNOGENICITY OF ANTIGEN BOUND TO THE PLASMA MEMBRANE OF MACROPHAGES
THE IMMUNOGENICITY OF ANTIGEN BOUND TO THE PLASMA MEMBRANE OF MACROPHAGES
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巨噬细胞质膜上抗原的免疫原性
DOI：
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发表时间：
1970
影响因子：
15.3
通讯作者：
J. Cerottini
中科院分区：
文献类型：
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作者：
E. Unanue;J. Cerottini
在短暂暴露于放射性碘标记的钥孔血蓝蛋白后，将巨噬细胞培养数小时。巨噬细胞摄取的大部分血蓝蛋白被迅速分解代谢并从细胞中消除。少数分子长时间保留在细胞质膜上，不受内吞作用和卡吞作用的影响。通过观察低温下抗体片段与巨噬细胞的固定，鉴定了与质膜结合的这几个血蓝蛋白分子。膜结合抗原，这可以通过胰蛋白酶或EDTA去除，是大分子大小，但异质性。用抗体或胰蛋白酶体外处理巨噬细胞，可以消除小鼠对与活巨噬细胞结合的血蓝蛋白的大部分免疫应答。因此，血蓝蛋白与巨噬细胞结合的免疫原性主要归因于与质膜结合的抗原分子较少。
Macrophages were cultured for several hours after a brief exposure to radio-iodinated keyhole limpet hemocyanin. Most of the hemocyanin taken up by the macrophages was rapidly catabolized and eliminated from the cell. A few molecules were retained on the plasma membrane of the cells for prolonged periods and were not subject to endocytosis and catabolism. These few molecules of hemocyanin bound to the plasma membrane were identified by observing the fixation of antibody fragments to macrophages at low temperature. The membrane-bound antigen, which could be removed by trypsin or EDTA, was of large molecular size, though heterogeneous. A great part of the immune responses of mice to hemocyanin bound to live macrophages could be abrogated by treatment of the macrophages in vitro with antibody or trypsin. Hence, most of the immunogenicity of hemocyanin bound to macrophages was attributed to the few molecules of antigen bound to the plasma membrane.