Studies on calf diarrhoea in Mozambique:: Prevalence of bacterial pathogens
Studies on calf diarrhoea in Mozambique:: Prevalence of bacterial pathogens
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DOI：
10.1186/1751-0147-45-27
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发表时间：
2004-01-01
影响因子：
2.1
通讯作者：
Möllby, R
中科院分区：
文献类型：
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作者：
Achá, SJ;Kühn, I;Möllby, R
在连续两年期间，对莫桑比克8个奶牛场的犊牛腹泻流行率进行了4次调查。在这些农场饲养了1241头6个月以下的小牛，其中63头（5%）出现腹泻症状。两个农场的腹泻总体患病率较高（13%和21%）。收集了所有腹泻犊牛（63头）和330头健康犊牛的粪便样本，并分析了沙门氏菌、空肠弯曲杆菌和产肠毒素大肠杆菌（ETEC）。沙门氏菌仅在2%的犊牛中被分离出来。弯曲杆菌在所有犊牛中分离出11%，无论其健康状况如何，并且在2个腹泻农场之一中更为常见（25%）（p=0.001）。80%的分离株鉴定为空肠杆菌。55株腹泻犊牛中未检出ETEC菌株，但22/55株（40%）腹泻犊牛和14/88株（16%）健康犊牛携带K99粘连素（p=0.001）。用生化指纹法（PhenePlate(TM)）对6757株分离的大肠杆菌进行了分型，发现健康犊牛和腹泻犊牛的大肠杆菌多样性相同。于是得出结论:i)腹泻的总体发病率很低,但2农场有较高的患病率,可能是由于爆发情况,2)沙门氏菌不似乎与腹泻,3)空肠弯曲杆菌是常见的2腹泻农场和iv) ETEC菌株没有发现,但K99抗原是在大肠杆菌菌株从腹泻小腿比健康,以及更普遍在一个腹泻农场。
The prevalence of diarrhoea in calves was investigated in 8 dairy farms in Mozambique at 4 occasions during 2 consecutive years. A total of 1241 calves up to 6 months of age were reared in the farms, and 63 (5%) of them had signs of diarrhoea. Two farms had an overall higher prevalence (13% and 21%) of diarrhoea. Faecal samples were collected from all diarrhoeal calves (n=63) and from 330 healthy calves and analysed for Salmonella species, Campylobacter jejuni and enterotoxigenic Escherichia coli (ETEC). Salmonella spp. was isolated in only 2% of all calves. Campylobacter was isolated in 11% of all calves, irrespective of health condition, and was more frequent (25%) in one of the 2 diarrhoeal farms (p=0.001). 80% of the isolates were identified as C.jejuni. No ETEC strains were detected among the 55 tested strains from diarrhoeal calves, but 22/55 (40%) strains from diarrhoeal calves and 14/88 (16%) strains from healthy calves carried the K99 adhesin (p=0.001). 6,757 E. coli isolates were typed with a biochemical fingerprinting method (the PhenePlate(TM)) giving the same E. coli diversity in healthy and diarrhoeal calves. Thus it was concluded: i) the overall prevalence of diarrhoea was low, but 2 farms had a higher prevalence that could be due to an outbreak situation, ii) Salmonella did not seem to be associated with diarrhoea, iii) Campylobacter jejuni was common at one of the 2 diarrhoeal farms and iv) ETEC strains were not found, but K99 antigen was more prevalent in E. coli strains from diarrhoeal calves than from healthy, as well as more prevalent in one diarrhoeal farm.