Establishing intracranial brain tumor xenografts with subsequent analysis of tumor growth and response to therapy using bioluminescence imaging.
Establishing intracranial brain tumor xenografts with subsequent analysis of tumor growth and response to therapy using bioluminescence imaging.
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DOI：
10.3791/1986
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发表时间：
2010-07-13
期刊：
影响因子：
--
通讯作者：
James, C David
中科院分区：
文献类型：
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作者：
Ozawa, Tomoko;James, C David
多年来，使用人脑肿瘤细胞的移植模型在神经肿瘤学研究中发挥了重要作用。在过去，建立人类肿瘤异种移植最常用的程序是从培养瓶中收集细胞，然后将收集的细胞注射到免疫缺陷小鼠的皮下。虽然这种方法在许多实验室仍然经常使用，但在过去的十年里，重点已经显著转向原位异种移植的建立，在脑肿瘤的情况下，这需要将肿瘤细胞注射到适当的神经解剖结构中。由于颅内异种移植的建立不能通过直接测量(如使用卡尺)来监测肿瘤的生长，因此重点转向原位脑肿瘤异种移植模型，因此有必要利用非侵入性成像来评估宿主动物的肿瘤负担。在目前可用的成像方法中，生物发光监测通常被认为是灵敏度、便利性和成本的最佳组合。在这里，我们将演示建立原位脑肿瘤的程序，以及在测试实验疗法时监测肿瘤生长和治疗反应的程序。
Transplantation models using human brain tumor cells have served an essential function in neuro-oncology research for many years. In the past, the most commonly used procedure for human tumor xenograft establishment consisted of the collection of cells from culture flasks, followed by the subcutaneous injection of the collected cells in immunocompromised mice. Whereas this approach still sees frequent use in many laboratories, there has been a significant shift in emphasis over the past decade towards orthotopic xenograft establishment, which, in the instance of brain tumors, requires tumor cell injection into appropriate neuroanatomical structures. Because intracranial xenograft establishment eliminates the ability to monitor tumor growth through direct measurement, such as by use of calipers, the shift in emphasis towards orthotopic brain tumor xenograft models has necessitated the utilization of non-invasive imaging for assessing tumor burden in host animals. Of the currently available imaging methods, bioluminescence monitoring is generally considered to offer the best combination of sensitivity, expediency, and cost. Here, we will demonstrate procedures for orthotopic brain tumor establishment, and for monitoring tumor growth and response to treatment when testing experimental therapies.