Restraint Stress Fails to Render C57BL/6 Mice Susceptible to Theiler's Virus-Induced Demyelination
Restraint Stress Fails to Render C57BL/6 Mice Susceptible to Theiler's Virus-Induced Demyelination
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DOI：
10.1159/000258694
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Welsh, C. Jane R.
中科院分区：
文献类型：
--
作者：
Steelman, Andrew J.;Alford, Eric;Welsh, C. Jane R.
目的：多发性硬化症是一种中枢神经系统退行性疾病，其病理机制与免疫调节相一致。虽然其病因尚不清楚，但人们认为多种因素都会影响该病的发病和恶化，包括遗传背景和环境因素。方法：我们感兴趣的是心理应激在泰勒氏病毒诱导的脱髓鞘病(TVID)发病和加重中的作用，TVID是一种病毒持续促进脱髓鞘的MS小鼠模型。在目前的研究中，我们确定了慢性束缚应激(RS)诱导的免疫抑制是否会导致正常抗TVID的C57BL/6小鼠建立持续的中枢神经系统感染，从而导致脱髓鞘。结果：我们的数据表明，重复7天的RS不足以导致病毒特异性获得性免疫下降，也不会显著改变CNS病毒水平。此外，持续到感染后4周的慢性重复RS既不改变病毒特异性Ig G的发展，也不改变旋转棒分析所确定的运动功能。此外，应激小鼠中枢神经系统的组织学分析表明，在感染后第193天，没有炎症或脱髓鞘。结论：应激本身不足以克服C57BL/6小鼠品系对TVID的遗传抗性。版权所有(C)2009 S.Karger AG，巴塞尔
Objectives: Multiple sclerosis is a degenerative disease of the CNS with a pathology consistent with immunological mediation. Although its cause is unknown, multiple factors are thought to influence both the onset and exacerbation of the disease, including both genetic background as well as environmental factors. Methods: We are interested in the effect of psychological stress on the onset and exacerbation of Theiler's virus-induced demyelinating disease (TVID), a murine model of MS in which viral persistence facilitates demyelination. In the current study, we determined whether chronic restraint stress (RS)-induced immunosuppression could result in the establishment of a persistent CNS infection in the normally TVID-resistant C57BL/6 mouse strain, resulting in demyelination. Results: Our data indicated that RS repeated over the course of 7 days was not sufficient to cause decreases in virus-specific adaptive immunity, and did not significantly alter CNS viral levels. Furthermore, chronic repeated RS lasting until 4 weeks after infection altered neither the development of virus-specific IgG nor motor function determined by Rotarod analysis. In addition, histological analysis of the CNS of stressed mice indicated no inflammation or demyelination on day 193 after infection. Conclusion: These results suggest that stress alone is not sufficient to overcome genetic resistance to TVID in the C57BL/6 mouse strain. Copyright (C) 2009 S. Karger AG, Basel