Smart PEGylated gold nanoparticles for the cytoplasmic delivery of siRNA to induce enhanced gene silencing
Smart PEGylated gold nanoparticles for the cytoplasmic delivery of siRNA to induce enhanced gene silencing
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DOI：
10.1246/cl.2006.1046
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发表时间：
2006-09-05
影响因子：
1.6
通讯作者：
Nagasaki, Yukio
中科院分区：
文献类型：
--
作者：
Oishi, Motoi;Nakaogami, Junpei;Nagasaki, Yukio
通过聚乙二醇-嵌段聚(2-（N，N-二甲氨基）甲基丙烯酸乙酯共聚物（PEG-PAMA）与金纳米粒子（GNPs）的络合，将siRNA固定在巯基（SH-siRNA）上，建立了siRNA的细胞质递送体系。含有SH-siRNA的聚乙二醇化GNPs在HuH-7细胞中显示出显著的RNAi活性。
A novel cytoplasmic delivery system of siRNA was developed by complexation between poly(ethylene glycol)-blockpoly(2-(N,N-dimethylamino)ethyl methacrylate) copolymer (PEG-PAMA) and gold nanoparticles (GNPs), followed by the immobilization of siRNA bearing a thiol group (SH-siRNA). The PEGylated GNPs containing SH-siRNA showed a significant RNAi activity in HuH-7 cells.