Determination of Free and Total Insulin and C-Peptide in Insulin-treated Diabetics
Determination of Free and Total Insulin and C-Peptide in Insulin-treated Diabetics
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胰岛素治疗的糖尿病患者中游离胰岛素和总胰岛素及 C 肽的测定
DOI：
10.2337/diab.26.1.22
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发表时间：
1977
期刊：
影响因子：
7.7
通讯作者：
A. Rubenstein
中科院分区：
文献类型：
--
作者：
H. Kuzuya;P. Blix;D. Horwitz;D. Steiner;A. Rubenstein
对胰岛素抗体阳性糖尿病患者酸化前后血清游离胰岛素、总胰岛素水平及总C肽免疫反应性(C-肽、胰岛素原)和C-肽进行了测定。聚乙二醇乙二醇酯使胰岛素抗体从血清中完全沉淀下来，并使测定上清液中的游离胰岛素成为可能。在加入聚乙二醇前，将血清在37δC下孵育两小时，得到的游离胰岛素值可能与体内水平最接近。同样的方法也可以用来去除与循环胰岛素抗体结合的胰岛素原，并允许测量上清液中的C-肽。临床研究表明，联合检测血清游离胰岛素、总胰岛素和C肽有助于了解内源性和外源性胰岛素在糖尿病患者病程和代谢控制中的作用。
Serum levels of free and total insulin as well as total C-peptide immunoreactivity (C-peptide and proinsulin) and C-peptide were measured in insulin-treated diabetics with circulating insulin antibodies by the addition of polyethylene glycol (PEG) before and after acidification. PEG resulted in complete precipitation of insulin antibodies from serum and made it possible to measure free insulin in the supernatant. Incubation of serum at 37δ C. for two hours before addition of PEG resulted in values for free insulin that probably resembled the in-vivo levels most closely. The same method could also be used to remove proinsulin bound to circulating insulin antibodies and permitted the measurement of C-peptide in the supernatant. Clinical studies using this approach indicate that combined measurements of serum free and total insulin and C-peptide provide information that is helpful in understanding the contribution of endogenous and exogenous insulin to the course and metabolic control of insulin-requiring diabetic patients.