RELATIONSHIP BETWEEN AGE AND GSH METABOLISM IN SYNAPTOSOMES OF RAT CEREBRAL-CORTEX
RELATIONSHIP BETWEEN AGE AND GSH METABOLISM IN SYNAPTOSOMES OF RAT CEREBRAL-CORTEX
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DOI：
10.1016/0197-4580(94)90074-4
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发表时间：
1994-07-01
影响因子：
4.2
通讯作者：
VINCENZINI, MT
中科院分区：
文献类型：
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作者：
FAVILLI, F;IANTOMASI, T;VINCENZINI, MT
本文对大鼠大脑皮层突触体谷胱甘肽代谢随年龄的变化进行了综合分析。所有不同的谷胱甘肽系统成分（GSH、GSSG、总GSH和GSH氧化还原指数）仅在衰老过程中发生显著变化。GSH、总GSH和GSH氧化还原指数分别下降了约40%、24%和52%，而GSSG则显著增加了约60%。相反，一些gsh相关酶活性在生长和衰老过程中都表现出特征性的变化。GSH过氧化物酶和GSH- s -转移酶活性在生长和衰老过程中均显著升高，GSH还原酶和γ -谷氨酰半胱氨酸合成酶活性仅在衰老过程中降低，而葡萄糖-6-磷酸脱氢酶活性在大鼠的整个生命过程中没有变化。所获得的结果表明，氧化状态的增加是由于突触体隔室中GSH系统抗氧化能力的降低。这些代谢改变的主要原因是GSSG还原成谷胱甘肽和合成谷胱甘肽的速率降低。此外，在衰老过程中，由于高度解毒机制，GSH作为GSH- s偶联物的不可逆损失也是可能的。这些主要在大鼠大脑皮层突触体衰老过程中发现的谷胱甘肽代谢的改变可能有助于阐明大脑疾病的某些方面。
A comprehensive analysis on glutathione metabolism in rat cerebral cortex synaptosomes as a function of age was performed. All different glutathione system components (GSH, GSSG, total GSH, and GSH redox index) changed significantly only during aging. GSH, total GSH, and GSH redox index decreased by about 40%, 24%, and 52%, respectively, while GSSG showed a remarkable increase of about 60%. On the contrary, some GSH-related enzyme activities showed characteristic changes both during growth and aging. GSH peroxidase and GSH-S-transferase activities significantly increased both during growth and aging, GSH reductase and gamma-glutamylcysteine synthetase activities showed lower levels only during aging, while glucose-6-phosphate dehydrogenase activity did not change throughout the life of the rat. The results obtained suggest an increase of the oxidative status due to a reduced antioxidant capacity of the GSH system in the synaptosomal compartment during aging. The main cause of these metabolic modifications is a lowering of the rates of both GSSG reduction to GSH and GSH synthesis. Moreover, an irreversible loss of GSH as GSH-S-conjugates due to a high detoxification mechanism during aging is also possible. These alterations in glutathione metabolism, found mainly during aging in rat cerebral cortex synaptosomes may contribute to clarify some aspects of cerebral diseases.