Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka
Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka
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DOI：
10.1155/2017/3196924
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Abeyewickreme, Wimaladharma
中科院分区：
文献类型：
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作者：
Gunathilaka, Nayana;Ranathunge, Tharaka;Abeyewickreme, Wimaladharma
人工膜饲养技术和血粉来源的选择已被公认为是媒介生物大量饲养的关键因素。方法论采用玻璃板、金属板和Hemotek膜饲养法3种人工膜饲养技术，以埃及伊蚊（Aedes aegypti）的摄食率、繁殖力和孵化率为指标，对人、牛和鸡3种血液来源进行了评价。通过单因素方差分析、方差聚类分析和主坐标分析，研究了摄食之间的差异。结果的取食率。单因素方差分析表明，不同膜饲养技术对埃及伊蚊的影响差异显著（p < 0.05）。金属板法被认为是最有效和最具成本效益的饲养技术。吸血率。埃及人血中的感染率较高，其次是牛血和鸡血。单因素方差分析表明，牛血和人血喂养的蚊虫在繁殖力、产卵率和生殖力方面无显著差异（p > 0.05）。结论.金属板法可作为规模化饲养的最有效的膜饲养技术。埃及，由于其高喂养率和成本效益。牛血可作为规模化养殖Ae的推荐血液。埃及人。
Selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors. Methodology. Artificial membrane feeding techniques, namely, glass plate, metal plate, and Hemotek membrane feeding method, and three blood sources (human, cattle, and chicken) were evaluated based on feeding rates, fecundity, and hatching rates of Aedes aegypti. Significance in the variations among blood feeding was investigated by one-way ANOVA, cluster analysis of variance (ANOSIM), and principal coordinates (PCO) analysis. Results. Feeding rates of Ae. aegypti significantly differed among the membrane feeding techniques as suggested by one-way ANOVA(p < 0.05). Themetal plate method was identified as the most efficient and cost-effective feeding technique. Blood feeding rate of Ae. aegypti was higher with human blood followed by cattle and chicken blood, respectively. However, no significant difference was observed from the mosquitoes fed with cattle and human blood, in terms of fecundity, oviposition rate, and fertility as suggested by one-way ANOVA (p > 0.05). Conclusions. Metal plate method could be recommended as the most effective membrane feeding technique for mass rearing of Ae. aegypti, due to its high feeding rate and cost effectiveness. Cattle blood could be recommended for mass rearing Ae. aegypti.