The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility.
The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility.
复制标题
myoA 基因编码的非常规肌球蛋白在盘基网柄菌的运动中发挥作用。
DOI：
10.1091/mbc.4.2.233
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发表时间：
1993
影响因子：
3.3
通讯作者：
Soll,D
中科院分区：
文献类型：
--
作者：
Titus,MA;Wessels,D;Spudich,JA;Soll,D
The myoA gene of Dictyostelium is a member of a gene family of unconventional myosins. The myosin Is share homologous head and basic domains, but the myoA gene product lacks the glycine-, proline-, alanine-rich and src homology 3 domains typical of several of the other myosin Is. A mutant strain of Dictyostelium lacking a functional myoA gene was produced by gene targeting, and the motility of this strain in buffer and a spatial gradient of the chemoattractant cyclic AMP was analyzed by computer-assisted methods. The myoA- cells have a normal elongate morphology in buffer but exhibit a decrease in the instantaneous velocity of cellular translocation, an increase in the frequency of lateral pseudopod formation, and an increase in turning. In a spatial gradient, in which the frequency of pseudopod formation is depressed, myoA- cells exhibit positive chemotaxis but still turn several times more frequently than control cells. These results demonstrate that the other members of the unconventional myosin family do not fully compensate for the loss of functional myoA gene product. Surprisingly, the phenotype of the myoA- strain closely resembles that of the myoB- strain, suggesting that both play a role in the frequency of pseudopod formation and turning during cellular translocation.
