Preparation of multiresponsive hydrophilic molecularly imprinted microspheres for rapid separation of gardenia yellow and geniposide from gardenia fruit
Preparation of multiresponsive hydrophilic molecularly imprinted microspheres for rapid separation of gardenia yellow and geniposide from gardenia fruit
复制标题
多响应亲水分子印迹微球的制备用于栀子果实中栀子黄和栀子苷的快速分离
DOI：
10.1016/j.foodchem.2021.131610
复制
发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
孟祥河
中科院分区：
文献类型：
--
作者：
王金双;叶沁;余宁翔;郇伟伟;孙井亮;聂小华;孟祥河
本文建立了一种基于分子印迹固相萃取（MISPE）技术分离栀子中栀子黄色素和栀子苷的方法。首先，以栀子黄为模板，采用可逆加成-断裂链转移（RAFT）沉淀聚合法制备了亲水性分子印迹微球。所得的HMIMs表现出对pH、温度和磁性的多响应性，实现了对栀子黄的可控吸收和释放，并且易于通过外部磁体回收。同时，HMIMs具有高吸附容量、快速结合动力学、特异性识别和可重复使用性。最后，通过对吸附和洗脱条件的优化，建立了以HMIMs为吸附剂的MISPE法提取栀子黄色素和纯化栀子苷的方法。栀子黄和栀子苷的相对纯度分别为99.77 ± 0.05%（回收率94.04 ± 0.10%）和94.50 ± 0.62%（回收率95.40 ± 0.86%）。
In this work, a robust method for the separation of gardenia yellow and geniposide from gardenia fruit was.developed based on a molecularly imprinted solid phase extraction (MISPE) procedure. First, hydrophilic.molecularly imprinted microspheres (HMIMs) were prepared using gardenia yellow as the template via reversible addition fragmentation chain transfer (RAFT) precipitation polymerization. The resultant HMIMs demonstrated the multiresponsiveness to pH, temperature, and magnetism, achieving controllable uptake and release of.gardenia yellow and easy recovery by external magnets. Meanwhile, the HMIMs possessed high adsorption capacity, fast binding kinetics, specific recognition, and reusability. Finally, a MISPE approach using HMIMs as.adsorbent was developed for extraction of gardenia yellow and purification of geniposide after optimization of.the adsorption and elution conditions. Thus, efficient separation of gardenia yellow and geniposide with relative.purities of 99.77 ± 0.05% (94.04 ± 0.10% recovered) and 94.50 ± 0.62% (95.40 ± 0.86% recovered),.respectively, was achieved.