Peroxidase-catalyzed coupling of phenol in the presence of model inorganic and organic solid phases
Peroxidase-catalyzed coupling of phenol in the presence of model inorganic and organic solid phases
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DOI：
10.1021/es049826h
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发表时间：
2004-10-01
影响因子：
11.4
通讯作者：
Weber, WJ
中科院分区：
文献类型：
--
作者：
Huang, QG;Weber, WJ
研究了在不同的石英砂、纤维素、木质素和聚甲基苯乙烯水溶液体系中，过氧化物酶催化苯酚的氧化偶联反应。这四种固相材料在其物理化学特性方面代表了广泛的不同天然地质吸附剂类型。每种固体被发现影响过氧化物酶催化的苯酚偶联，无论是通过减轻酶失活，通过参与交叉偶联，或通过这两种活动的组合。对于发现有效吸附酶的四种模型固体中的三种，观察到酶失活的减轻;即，纤维素、硅砂和木质素。两种固体，聚甲基苯乙烯和木质素，被发现参与显着的交叉偶联反应。据推测，相对亲水的固体可以通过形成酶-固体缔合来减轻过氧化物酶失活。芳香族结构或不饱和C-C键被认为是固相材料的特征，使它们能够参与交叉偶联。研究结果具有重要意义的过程可行性评估和工程设计的土壤/沉积物修复系统采用酶偶联方案。
Peroxidase-catalyzed oxidative coupling reactions of phenol in aqueous systems variously containing silica sand, cellulose, lignin, and polymethylstyrene were investigated. These four solid phase materials represent a broad spectrum of different natural geosorbent types in terms of their physicochemical characteristics. Each solid was found to influence peroxidase-catalyzed phenol coupling, either by mitigation of enzyme inactivation, by participation in cross-coupling, or by a combination of these two activities. Mitigation of enzyme inactivation was observed for those three of the four model solids found to adsorb the enzyme effectively; i.e., cellulose, silica sand, and lignin. Two solids, polymethylstyrene and lignin, were found to participate significantly in cross-coupling reactions. It is postulated that relatively hydrophilic solids can mitigate peroxidase inactivation by forming enzyme-solid associations. Aromatic structures or unsaturated C-C bonds were found to be features of the solid-phase materials that allowed them to participate in cross-coupling. The results have important implications for process feasibility assessment and the engineering design of soil/sediment remediation systems employing enzymatic coupling schemes.