Mechanobiology of cardiomyocyte development.
Mechanobiology of cardiomyocyte development.
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DOI：
10.1016/j.jbiomech.2009.09.014
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发表时间：
2010-01-05
影响因子：
2.4
通讯作者：
Hunt DL
中科院分区：
文献类型：
--
作者：
Jacot JG;Martin JC;Hunt DL
心肌细胞处于恒定的、自生的机械应力下，其可影响干细胞向心肌细胞的分化、分化细胞的发育和新生哺乳动物中细胞的成熟。本文综述了直接拉伸、电诱导搏动和基底弹性对心肌细胞行为和发育的影响，特别强调了基底刚度对心肌细胞成熟的影响。为了将这些观察结果与体内机械条件联系起来，我们分离了胚胎第13.5天至出生后第14天的瑞士黑小鼠的左心室，并使用原子力显微镜压痕测量了心外膜的弹性模量。我们发现心外膜的弹性模量在出生时发生显著变化，从胚胎的12 ± 4 kPa变化到新生儿的39 ± 7 kPa。这种变化在显示出显著影响新生心肌细胞发育的范围内。
Cardiac cells are under constant, self-generated mechanical stress which can affect the differentiation of stem cells into cardiac myocytes, the development of differentiated cells and the maturation of cells in neonatal mammals. In this article, the effects of direct stretch, electrically induced beating and substrate elasticity on the behavior and development of cardiomyocytes are reviewed, with particular emphasis on the effects of substrate stiffness on cardiomyocyte maturation. In order to relate these observations to in-vivo mechanical conditions, we isolated the left ventricle of Black Swiss mice from embryonic day 13.5 through postnatal day 14 and measured the elastic modulus of the epicardium using atomic force microscope indentation. We found that the elastic modulus of the epicardium significantly changes at birth, from an embryonic value of 12 ± 4 kPa to a neonatal value of 39 ± 7 kPa. This change is in the range shown to significantly affect the development of neonatal cardiomyocytes.
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