MR microscopy of multicomponent diffusion in single neurons
MR microscopy of multicomponent diffusion in single neurons
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DOI：
10.1002/mrm.1306
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发表时间：
2001-12-01
影响因子：
3.3
通讯作者：
Blackband, SJ
中科院分区：
文献类型：
--
作者：
Grant, SC;Buckley, DL;Blackband, SJ
这项研究考察了分离的单个神经元中的多组分扩散，并讨论了结果对组织中宏观水扩散的影响。L7海马神经元的分离和分析使用600 MHz Bruker大口径仪器和磁化率匹配的射频微线圈。采用双指数拟合法，快速组分的表观扩散系数(ADC)为0.034+/-0.14×10(-3)mm(2)，慢组分(N=10)的表观扩散系数为0.034+/-0.017×10(-3)mm(2)。核内的扩散主要是单指数扩散，但经双指数分析，快成分的扩散为1.31+/-0.32x10(-3)mm(2)，慢成分(N=5)为0.057+/-0.073×10(-3)mm(2)的S(-1)(89+/-6%)。胞核中的慢成分可以用胞浆体积平均来解释。这些数据表明，分离的单个海兔神经元胞浆内的水分扩散支持多指数模型。ADC与以前在单个神经元胞浆中的测量以及在脑灌流切片中的缓慢ADC测量是一致的。这些分布可能解释了在组织中观察到的多个间隔，极大地帮助了整个组织MRI定量模型的发展。(C)2001年Wiley-Liss，Inc.
This study examines multicomponent diffusion in isolated single neurons and discusses the implications of the results for macroscopic water diffusion in tissues. L7 Aplysia neurons were isolated and analyzed using a 600 MHz Bruker wide-bore instrument with a magnetic susceptibility-matched radiofrequency microcoil. Using a biexponential fit, the apparent diffusion coefficients (ADCs) from the cytoplasm (with relative fraction) were 0.48 +/- 0.14 x 10(-3) mm(2)S(-1) (61 +/- 11%) for the fast component, and 0.034 +/- 0.017 x 10(-3) mm(2)s(-1) (32 +/- 11 %) for the slow component (N = 10). Diffusion in the nucleus appears to be primarily monoexponential, but with biexponential analysis it yields 1.31 +/- 0.32 x 10(-3) mm(2)s(-1) (89 +/- 6%) for the fast component and 0.057 +/- 0.073 x 10(-3) mm(2)s(-1) (11 +/- 6%) for the slow (N = 5). The slow component in the nucleus may be explained by cytoplasmic volume averaging. These data demonstrate that water diffusion in the cytoplasm of isolated single Aplysia neurons supports a multiexponential model. The ADCs are consistent with previous measurements in the cytoplasm of single neurons and with the slow ADC measurement in perfused brain slices. These distributions may explain the multiple compartments observed in tissues, greatly aiding the development of quantitative models of MRI in whole tissues. (C) 2001 Wiley-Liss, Inc.