Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy
Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy
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DOI：
10.1111/j.1365-2818.2007.01873.x
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Pansu, R. B.
中科院分区：
文献类型：
--
作者：
Spitz, J. -A.;Yasukuni, R.;Pansu, R. B.
本文描述并表征了一种用于 FLIM 和荧光各向异性宽视野成像的无扫描单光子计数系统。荧光的两个偏振被格兰棱镜分开，并同时被 Q(A) 检测器采集。可以为任何所需区域（直至每个像素）重建荧光衰减曲线，并用于计算时间分辨荧光各向异性衰减。
A scanning-less single-photon counting system for FLIM and fluorescence anisotropy wide-field imaging is described and characterized in this paper. The two polarizations of the fluorescence are divided by a Glan prism and acquired at the same time by the Q(A) detector. Fluorescence decay profiles can be reconstructed for any desired area up to each pixel and used to calculate time-resolved fluorescence anisotropy decays.