An easily available ratiometric AIE probe for nitroxyl visualization in vitro and in vivo
An easily available ratiometric AIE probe for nitroxyl visualization in vitro and in vivo
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一种易于使用的比例 AIE 探针，用于体外和体内硝酰基可视化
DOI：
10.1039/d0qm00995d
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发表时间：
2021-02-21
影响因子：
7
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
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作者：
Li, Chunbin;Jiang, Guoyu;Tang, Ben Zhong
氮氧基(HNO)作为一种活性氮物种(RNS)，在许多生理和病理过程中起着关键作用，但由于缺乏高灵敏度和选择性的探针来实现HNO在生物系统中的实时显示和监测，内源性HNO的生成机制尚未阐明。比率荧光探针因其高灵敏度和抗干扰性而成为生物成像应用的有力平台。在这项工作中，我们首次开发了一种具有典型聚集诱导发射(AIE)特征的比率荧光探针(TCFPB-HNO)，用于HNO的检测和可视化，具有良好的光稳定性、高特异性和灵敏度。重要的是，TCFPB-HNO是通过两步工艺容易地高产率制造的，作为一个可用的AIE工具箱，可以用于实时监测活体样品中的HNO水平。
Nitroxyl (HNO), as a reactive nitrogen species (RNS), has a pivotal role in many physiological and pathological processes, but the generation mechanism of endogeneous HNO has not been elucidated, because of the lack of highly sensitive and selective probes to achieve real-time visualization and monitoring of HNO in biosystems. Ratiometric fluorescence probes have emerged as powerful platforms for bioimaging applications due to their high sensitivity and anti-interference ability. In this work, we developed firstly a ratiometric fluorescent probe (TCFPB-HNO) with typical aggregation-induced emission (AIE) characteristics for the detection and visualization of HNO with excellent photostability, high specificity and sensitivity. Importantly, TCFPB-HNO was facilely fabricated in high yields via a two-step process, as a serviceable AIE toolbox, which could be employed for real-time monitoring of the HNO level in live samples.