Selective protein adsorption and blood compatibility of hydroxy-carbonate apatites
Selective protein adsorption and blood compatibility of hydroxy-carbonate apatites
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DOI：
10.1002/jbm.a.30039
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发表时间：
2004-06-01
影响因子：
4.9
通讯作者：
Takashima, S
中科院分区：
文献类型：
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作者：
Takemoto, S;Kusudo, Y;Takashima, S
我们检查了羟基磷灰石和羟基碳酸盐磷灰石的血液相容性和蛋白质吸附性。这些磷灰石是在含0%、5%或15%CO2的N-2气氛下，用硝酸钙和磷酸氢二铵（摩尔比为5:3）的强氨碱溶液，通过湿化学法合成的，随后在105-700℃范围内煅烧。红外（IR）分析表明，羟基磷灰石晶格中碳酸根离子取代了磷酸根离子和羟基离子；磷酸盐离子和氢氧根离子的红外谱带强度在煅烧时降低。合成磷灰石的比表面积随着煅烧温度的升高而降低。根据活性部分凝血活酶时间、凝血酶原时间和与磷灰石接触的血浆的纤维蛋白原量来评估凝血特性，表明所有磷灰石几乎不影响凝血系统。将磷灰石与同时含有牛血清白蛋白（BSA）和β（2）-微球蛋白（β（2）-MG）的溶液接触，检查其上吸附的蛋白质的量：随着煅烧温度低于500℃，吸附的BSA和β（2）-MG的量逐渐增加，而当超过500℃时，吸附的BSA和β（2）-MG的量呈现突然增加。 600℃。在15%、含CO2的N-2气氛下合成并在400℃以下煅烧的羟基碳酸盐磷灰石对β(2)-MG的吸附具有最大的选择性。因此，β(2)-MG 吸附的较高选择性根据经验与羟基磷灰石晶格中掺入的碳酸根离子相关。 (C) 2004 年 Wiley 期刊公司。
We examined the blood compatibility and protein adsorption on hydroxyapatite and hydroxy-carbonate apatite. Those apatites were synthesized under a 0, 5, or 15% CO2-containing N-2 atmosphere by a wet-chemical method with a strong ammonia alkali solution of calcium nitrate and diammonium hydrogen phosphate (5:3 in molar ratio) and subsequent calcination in the range of 105-700degreesC. From infrared (IR) analysis, the carbonate ions substituted both phosphate ions and hydroxyl ions in the hydroxyapatite lattice; the intensities of IR bands assignable to phosphate ions and hydroxyl ions were reduced on calcinations. The specific surface areas of synthesized apatites decreased with increasing calcination temperature. Blood-clotting properties were evaluated in terms of active partial thromboplastin time, prothrombin time, and the amount of fibrinogen for the plasma in contact with the apatites, indicating that all the apatites barely influenced the blood clotting system. The apatites were in contact with a solution containing both bovine serum albumin (BSA) and beta(2)-microglobulin (beta(2)-MG), and the amounts of those proteins adsorbed on them were examined: the amount of absorbed BSA and beta(2)-MG gradually increased with the calcination temperature below 500degreesC, while it showed a sudden increase when more than 600degreesC. Hydroxy-carbonate apatite synthesized under a 15%, CO2-containing N-2 atmosphere and calcined below 400degreesC had the greatest selectivity in adsorbing beta(2)-MG. Thus, a higher selectivity for beta(2)-MG adsorption was empirically correlated to carbonate ions incorporated in the hydroxyapatite lattice. (C) 2004 Wiley Periodicals, Inc.