Construction of WCB-11: A novel phiYFP arsenic-resistant whole-cell biosensor
Construction of WCB-11: A novel phiYFP arsenic-resistant whole-cell biosensor
复制标题
WCB-11的构建：一种新型phiYFP抗砷全细胞生物传感器
DOI：
10.1016/s1001-0742(09)60277-1
复制
发表时间：
2010-01-01
影响因子：
6.9
通讯作者：
Zhuang, Guoqiang
中科院分区：
文献类型：
--
作者：
Hu, Qing;Li, Li;Zhuang, Guoqiang
环境砷污染预测与评价是倡导环境保护和开展人体健康风险评价的重要前提。构建了一种基于黄色荧光蛋白的全细胞亚砷酸盐和砷酸盐检测传感器。通过PCR从大肠杆菌DH5 α基因组中获得了一个抗砷启动子和调控基因arsR，并将phiYFP作为报告基因导入大肠杆菌DH5 α，构建了一个抗砷全细胞生物传感器（WCB-11）， phiYFP在该基因中首次得到良好表达。实验结果表明，该生物传感器对砷和phiYFP的表达有良好的响应。菌株WCB-11暴露于As3+和As5+时，黄色荧光的表达具有时间依赖性和剂量依赖性。这种工程结构有望成为一种廉价、方便的砷检测方法。
The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome of Escherichia coli DH5 alpha, and phiYFP was introduced into E. coli DH5 alpha as a reporter gene to construct an arsenic-resistant whole-cell biosensor (WCB-11) in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression of phiYFP. When strain WCB-11 was exposed to As3+ and As5+, the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field.