Trichoderma virens PDR-28: A heavy metal-tolerant and plant growth-promoting fungus for remediation and bioenergy crop production on mine tailing soil
Trichoderma virens PDR-28: A heavy metal-tolerant and plant growth-promoting fungus for remediation and bioenergy crop production on mine tailing soil
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DOI：
10.1016/j.jenvman.2013.10.009
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发表时间：
2014-01-01
影响因子：
8.7
通讯作者：
Oh, Byung-Taek
中科院分区：
文献类型：
--
作者：
Babu, A. Giridhar;Shim, Jaehong;Oh, Byung-Taek
从根际土壤中分离到一株耐重金属真菌Trichoderma virens PDR-28，并对其在矿山尾矿土壤修复和植物生物量生产中的应用进行了评价。PDR-28表现出植物生长促进特性，包括1-氨基环丙烷-1-羧酸（ACC）脱氨酶，酸性磷酸酶和植酸酶活性，铁载体的生产，和P溶解。接种PDR-28的尾矿土壤中重金属的有效性高于未接种的土壤，重金属的生物浸出顺序为Cd > As > Zn > Pb > Cu。PDR-28从含有100 mg HM L-1的液体培养基中有效去除HM，顺序为Pb > Cd > As > Zn > Cu。接种该菌后，玉米根系和地上部干生物量分别增加了64%和56%。叶绿素，总可溶性糖（可还原和不可还原），淀粉和蛋白质含量分别增加了46%，28%，30%和29%，相比，在未接种的土壤中生长的植物。接种玉米根中重金属浓度增加25%（铜）至62%（镉）和35%（铜）至64%（铅）相比，未接种的植物在地上部。结果表明，PDR-28将有利于植物稳定和植物生物质生产，作为寻求可再生能源的生物燃料的潜在来源。(C)2013由Elsevier Ltd.出版
A heavy metal-tolerant fungus, Trichoderma virens PDR-28, was isolated from rhizosphere soil and evaluated for use in remediating mine tailing soil and for plant biomass production. PDR-28 exhibited plant growth-promoting traits, including 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, acid phosphatase and phytase activity, siderophore production, and P solubilization. HMs were more available in mine tailing soil inoculated soil with PDR-28 than in uninoculated soil; the order of HM bioleaching was Cd > As > Zn > Pb > Cu. PDR-28 effectively removed HMs in the order of Pb > Cd > As > Zn > Cu from liquid media containing 100 mg HM L-1. Inoculating HM-contaminated mine tailing soil with the fungus significantly increased the dry biomass of maize roots (64%) and shoots (56%). Chlorophyll, total soluble sugars (reducible and nonreducible), starch, and protein contents increased by 46%, 28%, 30%, and 29%, respectively, compared to plants grown in uninoculated soil. Inoculation increased heavy metal concentrations in maize roots by 25% (Cu) to 62% (Cd) and in shoots by 35% (Cu) to 64% (Pb) compared to uninoculated plants. Results suggest that PDR-28 would be beneficial for phytostabilization and plant biomass production as a potential source of biofuel in the quest for renewable energy. (C) 2013 Published by Elsevier Ltd.