Intravital imaging strategy FlyVAB reveals the dependence of Drosophila enteroblast differentiation on the local physiology.
Intravital imaging strategy FlyVAB reveals the dependence of Drosophila enteroblast differentiation on the local physiology.
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活体成像策略 FlyVAB 揭示了果蝇肠母细胞分化对局部生理的依赖性。
DOI：
10.1038/s42003-021-02757-z
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发表时间：
2021-10-25
影响因子：
5.9
通讯作者：
Guo Z
中科院分区：
文献类型：
--
作者：
Tang R;Qin P;Liu X;Wu S;Yao R;Cai G;Gao J;Wu Y;Guo Z
果蝇肠道老化或损伤促进肠道干细胞（ISC）增殖和肠母细胞（EB）分化。然而，传统的谱系追踪方法评估局部生理学与动态EB分化的耦合方式仍然是模糊的。因此，我们开发了一种用于活体成像策略的3D打印平台“FlyVAB”，该平台能够在腹侧腹部角质层的单细胞水平上可视化果蝇后中肠。使用年轻和健康中肠中的ISC和年龄相关增生中肠中的肠内分泌细胞作为参考坐标，我们用Notch信号转导报告子追踪ISC-EB-肠上皮细胞谱系多日。我们的研究结果揭示了“分化平衡”EB状态与缓慢的ISC分裂和“分化激活”EB状态与ISC增生和快速EB肠上皮细胞分化相关。我们的FlyVAB成像策略为肠上皮的长时间活体成像打开了大门。
Aging or injury inDrosophilaintestine promotes intestinal stem cell (ISC) proliferation and enteroblast (EB) differentiation. However, the manner the local physiology couples with dynamic EB differentiation assessed by traditional lineage tracing method is still vague. Therefore, we developed a 3D-printed platform “FlyVAB” for intravital imaging strategy that enables the visualization of theDrosophilaposterior midgut at a single cell level across the ventral abdomen cuticle. Using ISCs in young and healthy midgut and enteroendocrine cells in age-associated hyperplastic midgut as reference coordinates, we traced ISC-EB-enterocyte lineages with Notch signaling reporter for multiple days. Our results reveal a “differentiation-poised” EB status correlated with slow ISC divisions and a “differentiation-activated” EB status correlated with ISC hyperplasia and rapid EB to enterocyte differentiation. Our FlyVAB imaging strategy opens the door to long-time intravital imaging of intestinal epithelium.