Single-molecule analysis of telomerase structure and function.
Single-molecule analysis of telomerase structure and function.
复制标题
DOI：
10.1016/j.cbpa.2011.10.008
复制
发表时间：
2011-12
影响因子：
7.8
通讯作者：
Stone MD
中科院分区：
文献类型：
--
作者：
Hengesbach M;Akiyama BM;Stone MD
The telomerase ribonucleoprotein is a specialized reverse transcriptase required to maintain protective chromosome end-capping structures called telomeres. In most cells, telomerase is not active and the natural shortening of telomeres with each round of DNA replication ultimately triggers cell growth arrest. In contrast, the presence of telomerase confers a high level of renewal capacity upon rapidly dividing cells. Telomerase is aberrantly activated in 90% of human cancers and thus represents an important target for anticancer therapeutics. However, the naturally low abundance of telomerase has hampered efforts to obtain high-resolution models for telomerase structure and function. To circumvent these challenges, single molecule techniques have recently been employed to investigate telomerase assembly, structure, and catalysis.
