Genetic control of serum antibody responses of inbred mice to type 1 and type 2 fimbriae from Actinomyces viscosus T14V.
Genetic control of serum antibody responses of inbred mice to type 1 and type 2 fimbriae from Actinomyces viscosus T14V.
复制标题
近交小鼠对粘性放线菌 T14V 1 型和 2 型菌毛的血清抗体反应的遗传控制。
DOI：
10.1128/iai.59.7.2364-2369.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Clark,WB
中科院分区：
文献类型：
--
作者：
Haber,J;Grinnell,CM;Beem,JE;Clark,WB
粘胶放线菌T14V中与1型和2型菌毛反应的抗体特异性地抑制粘胶放线菌T14V对唾液膜覆盖的牙齿表面和其他细菌的粘附，这些抗体被认为可以调节粘胶放线菌的定植。这些研究是为了确定近亲繁殖小鼠对1型和2型菌毛的抗体反应是否存在先前注意到的差异，可能是受遗传控制的。采用酶联免疫吸附法检测黏胶粘虫T14V细胞免疫的近交系、F1杂交和H-2基因小鼠血清免疫球蛋白G （IgG）和IgM抗体的反应，检测与黏胶粘虫T14V全细胞1型和2型菌毛反应的抗体。结果证实了早期的发现，并表明IgG抗1型抗体（23倍）和抗2型抗体（48倍）的数量发生了显著变化。17个自交系的反应从高到低呈相对连续的分布，H-2和Igh-C相同菌株对的反应差异显著。对F1杂交小鼠和H-2同源小鼠的应答分析表明，低应答基因(s)占主导地位，H-2连锁基因控制。来自两个高反应菌株的抗血清比来自低反应菌株的抗血清在体外抑制细菌粘附的程度要大得多。这些数据表明，与H-2相关的基因以及与H-2或high - c位点无关的背景基因可以多基因控制IgG抗1型和抗2型膜抗体反应的大小。
Antibodies reactive with type 1 and type 2 fimbriae from Actinomyces viscosus T14V specifically inhibit the adherence of A. viscosus T14V to salivary pellicle-coated tooth surfaces and other bacteria, and these antibodies are thought to modulate colonization by this microorganism. These studies were done to determine whether previously noted differences in the antibody responses of inbred mice to type 1 and type 2 fimbriae might be under genetic control. The serum immunoglobulin G (IgG) and IgM antibody responses of inbred, F1 hybrid, and H-2 congenic mice, immunized with A. viscosus T14V cells, were analyzed by enzyme-linked immunosorbent assays for antibodies reactive with A. viscosus T14V whole-cell type 1 and type 2 fimbriae. The results confirmed earlier findings and indicated striking variations in the amounts of IgG anti-type 1 (23-fold) and anti-type 2 (48-fold) fimbria antibodies elicited. The responses of the 17 inbred strains tested showed a relatively continuous distribution from high to low, as well as marked differences in the responses of H-2 and Igh-C identical strain pairs. An analysis of the responses of F1 hybrid and H-2 congenic mice indicated dominance of the low-responder gene(s) and control by H-2-linked genes. Antisera from two high-responder strains inhibited in vitro bacterial adherence to a much greater degree than antisera from a low-responding strain. These data suggest polygenic control of the magnitude of the IgG anti-type 1 and anti-type 2 fimbria antibody responses by H-2-linked genes as well as background genes not associated with H-2 or Igh-C loci.