Long noncoding RNA OIP5-AS1 acts as a competing endogenous RNA to promote glutamine catabolism and malignant melanoma growth by sponging miR-217
Long noncoding RNA OIP5-AS1 acts as a competing endogenous RNA to promote glutamine catabolism and malignant melanoma growth by sponging miR-217
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长非编码 RNA OIP5-AS1 充当竞争性内源 RNA，通过海绵 miR-217 促进谷氨酰胺分解代谢和恶性黑色素瘤生长
DOI：
10.1002/jcp.28335
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发表时间：
2019-09-01
影响因子：
5.6
通讯作者：
Bu, Xuefeng
中科院分区：
文献类型：
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作者：
Luan, Wenkang;Zhang, Xuanfeng;Bu, Xuefeng
长链非编码RNA（lncRNA）OIP 5-AS 1被认为促进许多人类肿瘤的生长和转移。然而，OIP 5-AS 1在黑色素瘤中的作用尚未报道。在这项研究中，我们发现OIP 5-AS 1水平在黑色素瘤组织中显著升高，并且OIP 5-AS 1高表达是黑色素瘤患者生存不良的独立风险因素。miR-217通过靶向谷氨酰胺酶（GLS）（谷氨酰胺催化剂的限速酶）抑制黑素瘤细胞中的谷氨酰胺催化剂。我们还证明了OIP 5-AS 1作为miR-217的海绵上调GLS表达，从而促进谷氨酰胺催化剂和黑色素瘤生长。总之，这一结果阐明了OIP 5-AS 1在黑色素瘤中代谢的新机制，并为黑色素瘤患者提供了潜在的治疗靶点。
The long noncoding RNA (lncRNA) OIP5-AS1 has been considered to promote the growth and metastasis of many human tumors. However, the role of OIP5-AS1 in melanoma has not been reported. In this study, we found that OIP5-AS1 levels were significantly elevated in melanoma tissue and that high OIP5-AS1 expression was an independent risk factor for the poor survival of patients with melanoma. miR-217 suppressed glutamine catabolism in melanoma cells by targeting glutaminase (GLS), the rate-limiting enzyme of glutamine catabolism. We also demonstrated that OIP5-AS1 acted as a sponge of miR-217 to upregulate GLS expression, thus promoting glutamine catabolism and melanoma growth. Overall, this result elucidates a new mechanism for OIP5-AS1 in metabolism in melanoma and provides a potential therapeutic target for patients with melanoma.