Anti-inflammatory activity and structural identification of a sulfated polysaccharide CLGP4 from Caulerpa lentillifera
Anti-inflammatory activity and structural identification of a sulfated polysaccharide CLGP4 from Caulerpa lentillifera
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来自 Caulerpa lentillifera 的硫酸化多糖 CLGP4 的抗炎活性和结构鉴定。
DOI：
10.1016/j.ijbiomac.2019.09.216
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发表时间：
2020-03-01
影响因子：
8.2
通讯作者：
Chen, Xuefeng
中科院分区：
文献类型：
--
作者：
Sun, Yujiao;Liu, Zhengqi;Chen, Xuefeng
本研究对从食用绿藻中提取的四种纯化多糖CLGP1、CLGP2、CLGP3和CLGP4的体外抗炎活性进行了评价。结果表明，CLGP4对lps诱导的HT29细胞表现出更有效的抑制作用，包括减少IL-113、TNF-a、SIgA和mucin2的产生，降低IL-10和TNF-a的表达。结果表明，CLGP4具有较好的抗炎作用，可能与硫酸基团的存在密切相关。通过甲基化分析、气相色谱-质谱分析和核磁共振谱分析CLGP4的结构。结果表明，CLGP4是一种由4)- man…- 2,4)Manp(1-。[j], 641 (2)- mang, 0-(13)- gale, (3-(74)- xylp，末端13-Gale和末端B-Xylp残基。此外，硫酸盐基团位于- 0.4)Xylp（1-）的C-3上。， C-6的- 3)Galp(1-。和C-3的- 0.2)Manp(1-…这些结果可以扩大CLGP4作为功能成分在减轻炎症中的潜在应用。(C) 2019 Elsevier B.V.版权所有
In the present study, the in vitro anti-inflammatory activity of four purified polysaccharides (CLGP1, CLGP2, CLGP3 and CLGP4) extracted from edible green algae Caulerpa lentillifera was evaluated. As a result, CLGP4 exhibited more effectively inhibitory effect on LPS-induced HT29 cells, including reducing the production of IL-113, TNF-a, SIgA and mucin2, and decreasing the expression of IL-10 and TNF-a. According to the results, CLGP4 showed a better anti-inflammatory effect, might highly related to the presence of sulfate groups. Furthermore, the structure of CLGP4 was analyzed by methylation analysis, GC-MS and NMR spectroscopy. It was found that CLGP4 was a novel xylogalactomanan consisting of 4)-Man,,, -.2,4)Manp(1-., 641 2)-Mang, 0-(1 3)-Gale, (3-(7 4)-Xylp, terminal 13-Gale and terminal B-Xylp residues. Additionally, the sulfate groups were located on C-3 of -.4)Xylp(1-., C-6 of -.3) Galp(1-. and C-3 of -.2)Manp(1--.. These results could enlarge the potential application of CLGP4 as functional ingredient to attenuate inflammation. (C) 2019 Elsevier B.V. All rights reserved.