A recombinant measles vaccine expressing chikungunya virus-like particles is strongly immunogenic and protects mice from lethal challenge with chikungunya virus
A recombinant measles vaccine expressing chikungunya virus-like particles is strongly immunogenic and protects mice from lethal challenge with chikungunya virus
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DOI：
10.1016/j.vaccine.2013.05.086
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发表时间：
2013-08-12
期刊：
影响因子：
5.5
通讯作者：
Tangy, Frederic
中科院分区：
文献类型：
--
作者：
Brandler, Samantha;Ruffle, Claude;Tangy, Frederic
基孔肯雅病毒（CHIKV）是一种蚊子传播的甲病毒，最近在印度洋、印度和东南亚重新出现，造成数百万例严重多关节痛病例。目前还没有预防疾病的专门治疗方法或限制流行病的疫苗。在这里，我们描述了一种重组减毒麻疹活疫苗（MV），该疫苗表达了来自最近的CHIKV菌株La Reunion的由衣壳和包膜结构蛋白组成的CHIKV病毒样颗粒。对麻疹病毒敏感的小鼠进行免疫接种可产生高滴度的CHIKV抗体，这些抗体可中和若干原代分离株。特异性细胞免疫反应也被激发。单次免疫该候选疫苗可保护所有小鼠免受致命的CHIKV攻击，并且免疫血清的被动转移对幼稚小鼠也具有保护作用。麻疹疫苗是最安全、最有效的人类疫苗之一。重组MV-CHIKV病毒可以制成一种安全有效的基孔肯雅热疫苗，值得在人体试验中进一步测试。(C) 2013 Elsevier Ltd.版权所有。
Chikungunya virus (CHIKV), a mosquito-transmitted alphavirus, recently reemerged in the Indian Ocean, India and Southeast Asia, causing millions of cases of severe polyarthralgia. No specific treatment to prevent disease or vaccine to limit epidemics is currently available. Here we describe a recombinant live-attenuated measles vaccine (MV) expressing CHIKV virus-like particles comprising capsid and envelope structural proteins from the recent CHIKV strain La Reunion. Immunization of mice susceptible to measles virus induced high titers of CHIKV antibodies that neutralized several primary isolates. Specific cellular immune responses were also elicited. A single immunization with this vaccine candidate protected all mice from a lethal CHIKV challenge, and passive transfer of immune sera conferred protection to naive mice. Measles vaccine is one of the safest and most effective human vaccines. A recombinant MV-CHIKV virus could make a safe and effective vaccine against chikungunya that deserves to be further tested in human trials. (C) 2013 Elsevier Ltd. All rights reserved.