ADSORPTION-KINETICS OF LATERALLY AND POLARLY FLAGELLATED VIBRIO
ADSORPTION-KINETICS OF LATERALLY AND POLARLY FLAGELLATED VIBRIO
复制标题
DOI：
10.1128/jb.151.3.1568-1580.1982
复制
发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
COLWELL, RR
中科院分区：
文献类型：
--
作者：
BELAS, MR;COLWELL, RR
测定了侧向和极性鞭毛细菌对甲壳素的吸附，并根据所得数据，推导出了修正的Langmuir吸附等温线。结果表明，侧鞭毛的副溶血性弧菌的吸附遵循朗缪尔吸附等温线，一种类型的吸附被称为表面饱和动力学，当条件有利于侧鞭毛的生产。当条件不利于侧鞭毛的产生时，细菌吸附不遵循Langmuir吸附等温线;相反，观察到成比例的吸附动力学。一些极性鞭毛细菌的吸附表现为表面饱和动力学。然而，极性鞭毛细菌的结合指数（结合位点的数量和细菌对表面的亲和力的乘积）与横向鞭毛细菌的结合指数显著不同，这表明极性鞭毛细菌通过与横向鞭毛细菌所使用的机制不同的机制吸附到几丁质上。从双标记吸附竞争实验的结果，其中极性鞭毛的霍乱弧菌与增加浓度的侧向鞭毛的副溶血性弧菌竞争，观察到侧向鞭毛的细菌抑制极性鞭毛的细菌的吸附。相反，极性鞭毛细菌增强了霍乱弧菌的吸附。在竞争实验中，副溶血性弧菌与浓度增加的其他细菌竞争，极性鞭毛细菌显著增强了副溶血性弧菌的吸附，而侧向鞭毛细菌仅略微增强了该过程。表面饱和动力学，侧鞭毛的生产，和横向鞭毛细菌的能力，以抑制极性鞭毛细菌的吸附之间观察到的直接相关性表明，侧鞭毛代表细菌结构的一个组成部分，这是重要的横向鞭毛细菌的吸附表面。提出了一个模型的吸附事件的横向鞭毛虫细菌，根据证据。
The adsorption of laterally and polarly flagellated bacteria to chitin was measured, and from the data obtained, a modified Langmuir adsorption isotherm was derived. Results indicated that the adsorption of laterally flagellated Vibrio parahaemolyticus follows the Langmuir adsorption isotherm, a type of adsorption referred to as surface saturation kinetics, when conditions are favorable for the production of lateral flagella. When conditions were not favorable for the production of lateral flagella, bacterial adsorption did not follow the Langmuir adsorption isotherm; instead, proportional adsorption kinetics were observed. The adsorption of some polarly flagellated bacteria exhibited surface saturation kinetics. However, the binding index (the product of the number of binding sites and bacterial affinity to the surface) of polarly flagellated bacteria differed significantly from that of laterally flagellated bacteria, suggesting that polarly flagellated bacteria adsorb to chitin by a different mechanism from that used by the laterally flagellated bacteria. From the results of dual-label adsorption competition experiments, in which polarly flagellated V. cholerae competed with increasing concentrations of laterally flagellated V. parahaemolyticus, it was observed that laterally flagellated bacteria inhibited the adsorption of polarly flagellated bacteria. In contrast, polarly flagellated bacteria enhanced the adsorption of V. cholerae. In competition experiments, where V. parahaemolyticus competed against increasing concentrations of other bacteria, polarly flagellated bacteria enhanced V. parahaemolyticus adsorption significantly, whereas laterally flagellated bacteria only slightly enhanced the process. The direct correlation observed between surface saturation kinetics, the production of lateral flagella, and the ability of laterally flagellated bacteria to inhibit the adsorption of polarly flagellated bacteria suggests that lateral flagella represent a component of bacterial structure that is important in the adsorption of laterally flagellated bacteria to surfaces. A model for adsorption events of laterally flagellated bacteria is proposed, based on the evidence presented.