EOBII Controls Flower Opening by Functioning as a General Transcriptomic Switch
EOBII Controls Flower Opening by Functioning as a General Transcriptomic Switch
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DOI：
10.1104/pp.111.176248
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发表时间：
2011-06-01
期刊：
影响因子：
7.4
通讯作者：
Clark, David G.
中科院分区：
文献类型：
--
作者：
Colquhoun, Thomas A.;Schwieterman, Michael L.;Clark, David G.
R2 R3-MYB转录因子（TF）参与植物生物学的各个方面。最近，在矮牵牛×杂交种系P720中鉴定了R2 R3-MYB，其在花挥发物产生的转录调节中起作用。我们提出R2 R3-MYB TF苯类化合物II（EOBII）的发射的更基础的作用。EOBII的同源物是从P. x hybrida 'Mitchell二倍体'（MD）和烟草（Nicotiana attenuata）中分离和鉴定的。对于MD和N。envelopata中，EOBII转录物在花组织中积累到高水平，在花开放时积累最大。当在MD和N. attenuata，ir-EOBII花不能进入开花期并过早衰老。转录物积累分析表明，在ir-EOBII花中，核心类苯丙素途径转录物和细胞壁修饰剂转录物水平改变。这些花可以部分补充喂养的蔗糖，t-肉桂酸，赤霉酸的解决方案;大概恢复细胞方面足够的花开放。此外，如果乙烯敏感性在MD或N中被阻断，attenuata，ir-EOBII花进入开花期。这些实验证明，一个R2 R3-MYB TF可以控制被子植物有性生殖的基本高度动态过程：花的开放。
R2R3-MYB transcription factors (TFs) are involved in diverse aspects of plant biology. Recently an R2R3-MYB was identified in Petunia x hybrida line P720 to have a role in the transcriptional regulation of floral volatile production. We propose a more foundational role for the R2R3-MYB TF EMISSION OF BENZENOIDS II (EOBII). The homolog of EOBII was isolated and characterized from P. x hybrida 'Mitchell Diploid' (MD) and Nicotiana attenuata. For both MD and N. attenuata, EOBII transcript accumulates to high levels in floral tissue with maximum accumulation at flower opening. When EOBII transcript levels are severely reduced using a stable RNAi (ir) approach in MD and N. attenuata, ir-EOBII flowers fail to enter anthesis and prematurely senesce. Transcript accumulation analysis demonstrated core phenylpropanoid pathway transcripts and cell wall modifier transcript levels are altered in ir-EOBII flowers. These flowers can be partially complemented by feeding with a sucrose, t-cinnamic acid, and gibberellic acid solution; presumably restoring cellular aspects sufficient for flower opening. Additionally, if ethylene sensitivity is blocked in either MD or N. attenuata, ir-EOBII flowers enter anthesis. These experiments demonstrate one R2R3-MYB TF can control a highly dynamic process fundamental to sexual reproduction in angiosperms: the opening of flowers.