AN INSERTION WITHIN A VARIABLY SPLICED DROSOPHILA TROPOMYOSIN GENE BLOCKS ACCUMULATION OF ONLY ONE ENCODED ISOFORM
AN INSERTION WITHIN A VARIABLY SPLICED DROSOPHILA TROPOMYOSIN GENE BLOCKS ACCUMULATION OF ONLY ONE ENCODED ISOFORM
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DOI：
10.1016/0092-8674(85)90061-3
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
FYRBERG, EA
中科院分区：
文献类型：
--
作者：
KARLIK, CC;FYRBERG, EA
一个异常等位基因的一个剪接的果蝇原肌球蛋白基因的特点。该等位基因是从不会飞的lfm（3）3品系中回收的，该品系具有结构和功能异常的间接飞行肌。该突变基因的转录部分被具有copia样果蝇移动的元件典型结构的8.8 kb插入的中间重复DNA中断。插入被定位成以一种剪接模式中断外显子序列，同时以交替模式中断内含子。作为插入的结果，该等位基因未能直接合成飞行肌特异性原肌溶素亚型，但仍然能够指定第二亚型，其在非原纤维果蝇肌肉中积累。
An aberrant allele of a variably spliced Drosophila tropomyosin gene was characterized. The allele was recovered from the flightless lfm(3)3 strain, which has structurally and functionally abnormal indirect flight muscles. The transcribed portion of the mutant gene is interrupted by an 8.8 kb [kilobase] insertion of middle repetitive DNA having a structure typical of copia-like Drosphila mobile elements. The insertion is positioned so as to interrupt an exon sequence in one splicing mode and, simultaneously, and intron in the alternate mode. As a consequence of the insertion the allele fails to direct synthesis of the flight muscle-specific tropomysin isoform, but remains capable of specifying a 2nd isoform, which accumulates in nonfibrillar Drosophila muscles.